Recombinant Human DNASE1 protein, His-tagged
Cat.No. : | DNASE1-1529H |
Product Overview : | Recombinant Human DNASE1 aa. (Gly19~Ala259) fused with N-terminal His tag was produced in E. coli cells. |
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Description : | This gene encodes a member of the DNase family. This protein is stored in the zymogen granules of the nuclear envelope and functions by cleaving DNA in an endonucleolytic manner. At least six autosomal codominant alleles have been characterized, DNASE1*1 through DNASE1*6, and the sequence of DNASE1*2 represented in this record. Mutations in this gene have been associated with systemic lupus erythematosus (SLE), an autoimmune disease. A recombinant form of this protein is used to treat the one of the symptoms of cystic fibrosis by hydrolyzing the extracellular DNA in sputum and reducing its viscosity. Alternate transcriptional splice variants of this gene have been observed but have not been thoroughly characterized. |
Source : | E. coli |
Species : | Human |
Tag : | His |
Form : | Freeze-dried powder |
Molecular Mass : | 28kDa as determined by SDS-PAGE reducing conditions. |
Protein length : | Gly19~Ala259 |
Endotoxin : | <1.0EU per 1µg (determined by the LAL method). |
Purity : | >92% |
Characteristic : | The isoelectric point is 4.6. |
Applications : | SDS-PAGE; WB; ELISA; IP; CoIP; Purification; Amine Reactive Labeling. |
Stability : | The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition. |
Storage : | Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months. |
Storage buffer : | PBS, pH7.4, containing 1mM DTT, 5% trehalose, 0.01% sarcosyl and Proclin300. |
Reconstitution : | Reconstitute in PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex. |
Gene Name : | DNASE1 deoxyribonuclease 1 [ Homo sapiens (human) ] |
Official Symbol : | DNASE1 |
Synonyms : | DNL1; DRNI; DNase I, lysosomal; Dornase alfa; deoxyribonuclease I; human urine deoxyribonuclease I; deoxyribonuclease-1 |
Gene ID : | 1773 |
mRNA Refseq : | NM_001351825.1 |
Protein Refseq : | NP_001338754.1 |
UniProt ID : | P24855 |
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◆ Native Protein | ||
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◆ Lysates | ||
DNASE1-866HCL | Recombinant Human DNASE1 cell lysate | +Inquiry |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (7)
Ask a questionDNASE1 helps degrade DNA in apoptotic cells, ensuring proper clearance and preventing inflammation.
DNASE1 deficiency can contribute to autoimmune diseases like SLE by allowing self-DNA accumulation.
DNASE1 regulates inflammation and immune responses by controlling extracellular DNA levels.
DNASE1 aids in resolving inflammation and promoting tissue repair by degrading DNA in damaged tissues.
DNASE1 maintains the stability and integrity of extracellular DNA, preventing inappropriate immune activation.
Changes in DNASE1 function can increase susceptibility to chronic inflammation and autoimmune disorders.
DNASE1 prevents the accumulation of self-DNA, reducing the risk of autoimmune reactions.
Customer Reviews (3)
Write a reviewDependable antibody validation, vital for our immunoprecipitation studies.
Top-quality protein purification service, essential for our lab's work.
Fast and reliable protein expression, a critical part of our projects.
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