Recombinant Human DNASE1 protein, His-tagged

• Cat.No.: DNASE1-3522H
• Product Overview: Recombinant Human DNASE1 protein(1-282 aa), fused to His tag, was expressed in E. coli.

Specification

• Source: E. coli
• Species: Human
• Tag: His
• Form: The purified protein was Lyophilized from sterile PBS (58mM Na2HPO4,17mM NaH2PO4, 68mM NaCl, pH7.4). 5 % trehalose and 5 % mannitol are added as protectant before lyophilization. The elution buffer contain 300mM imidazole.
• Protein length: 1-282 aa
• AA Sequence: MRGMKLLGALLALAALLQGAVSLKI AAFNIQTFGETKMSNATLVSYIVQI LSRYDIALVQEVRDSHLTAVGKLLD NLNQDAPDTYHYVVSEPLGRNSYKE RYLFVYRPDQVSAVDSYYYDDGCEP CGNDTFNREPAIVRFFSRFTEVREF AIVPLHAAPGDAVAEIDALYDVYLD VQEKWGLEDVMLMGDFNAGCSYVRP SQWSSIRLWTSPTFQWLIPDSADTT ATPTHCAYDRIVVAGMLLRGAVVPD SALPFNFQAAYGLSDQLAQAISDHY PVEVMLK
• Purity: 95%, by SDS-PAGE with Coomassie Brilliant Blue staining.
• Storage: Short-term storage: Store at 2-8°C for (1-2 weeks).
  Long-term storage: Aliquot and store at -20°C to -80°C for up to 3 months, buffer containing 50% glycerol is recommended for reconstitution. Avoid repeat freeze-thaw cycles.
• Reconstitution: Reconstitute at 0.25 µg/μl in 200 μl sterile water for short-term storage.
  Reconstitution with 200 μl 50% glycerol solution is recommended for longer term storage.

Gene Information

• Gene Name: DNASE1 deoxyribonuclease I [ Homo sapiens ]
• Official Symbol: DNASE1
• Synonyms: DNASE1; deoxyribonuclease I; DNL1; deoxyribonuclease-1; Dornase alfa; DNase I, lysosomal; human urine deoxyribonuclease I; DRNI; FLJ38093; FLJ44902; DKFZp686H0155
• Gene ID: 1773
• mRNA Refseq: NM_005223
• Protein Refseq: NP_005214
• MIM: 125505
• UniProt ID: P24855

Reviews

12/06/2022

Dependable antibody validation, vital for our immunoprecipitation studies.

01/10/2022

Top-quality protein purification service, essential for our lab's work.

02/22/2019

Fast and reliable protein expression, a critical part of our projects.

Q&As

How does DNASE1 (Deoxyribonuclease I) contribute to DNA degradation and clearance, particularly in apoptotic cells?

DNASE1 helps degrade DNA in apoptotic cells, ensuring proper clearance and preventing inflammation.

How does DNASE1 deficiency lead to the development of systemic lupus erythematosus (SLE) and other autoimmune diseases?

DNASE1 deficiency can contribute to autoimmune diseases like SLE by allowing self-DNA accumulation.

What is the impact of DNASE1 on the regulation of inflammation and immune system activation?

DNASE1 regulates inflammation and immune responses by controlling extracellular DNA levels.

How does DNASE1 influence the resolution of inflammation and tissue repair processes?

DNASE1 aids in resolving inflammation and promoting tissue repair by degrading DNA in damaged tissues.

What are the mechanisms by which DNASE1 modulates the stability and integrity of extracellular DNA?

DNASE1 maintains the stability and integrity of extracellular DNA, preventing inappropriate immune activation.

How do alterations in DNASE1 expression or activity affect susceptibility to chronic inflammatory conditions?

Changes in DNASE1 function can increase susceptibility to chronic inflammation and autoimmune disorders.

What role does DNASE1 play in preventing the accumulation of self-DNA and autoimmune responses?

DNASE1 prevents the accumulation of self-DNA, reducing the risk of autoimmune reactions.