Recombinant Human DNASE1L3 protein, GST-tagged

• Cat.No.: DNASE1L3-12097H
• Product Overview: Recombinant Human DNASE1L3 protein(NP_001243489.1)(21-305aa), fused to GST-tag, was expressed in E. coli.

Specification

• Description: The cDNA fragment encoding 21-305aa of Human Deoxyribonuclease gamma (DNASE1L3) was fused with an N-terminal GST-tag and then expressed in E.coli. The product obtained is the recombinant full-length of mature human DNASE1L3 protein. Its purity was determined by using SDS-PAGE and reached up to 90%. Under the reducing conditions, the gel presented a molecular mass band of about 62 kDa. The slightly higher result was attributed to glycosylation. It was also validated by the LC-MS/MS analysis. In-stock DNASE1L3 proteins are offered now. This recombinant DNASE1L3 protein may find uses in the specific antibody generation or the studies of cell biology.
  DNASE1L3 is a secreted DNASE1-like nuclease, which can digest DNA in chromatin. The deletion of DNASE1L3 can cause anti-DNA responses and autoimmunity in humans and mice. Lee Serpas et al. proved that DNASE1L3 plays a role in circulating plasma DNA homeostasis by enhancing fragmentation and affecting terminal motif frequencies. These results support the unique role of DNASE1L3 as a regulator of physical form and cell-free DNA availability, which may be significant for the DNASE1L3's mechanism of preventing autoimmunity.
• Source: E. coli
• Species: Human
• Tag: GST
• Form: Tris-based buffer,50% glycerol
• Molecular Mass: 60.4kDa
• Protein length: 21-305aa
• AA Sequence: MRICSFNVRSFGESKQEDKNAMDVI VKVIKRCDIILVMEIKDSNNRICPI LMEKLNRNSRRGITYNYVISSRLGR NTYKEQYAFLYKEKLVSVKRSYHYH DYQDGDADVFSREPFVVWFQSPHTA VKDFVIIPLHTTPETSVKEIDELVE VYTDVKHRWKAENFIFMGDFNAGCS YVPKKAWKNIRLRTDPRFVWLIGDQ EDTTVKKSTNCAYDRIVLRGQEIVS SVVPKSNSVFDFQKAYKLTEEEALD VSDHFPVEFKLQSSRAFTNSKKSVT LRKKTKSKRS
• Purity: Greater than 90% as determined by SDS-PAGE.
• Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
• Storage: Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
• Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Gene Information

• Gene Name: DNASE1L3 deoxyribonuclease 1 like 3 [ Homo sapiens (human) ]
• Official Symbol: DNASE1L3
• Synonyms: Deoxyribonuclease gamma; Deoxyribonuclease I like 3; Deoxyribonuclease I like III; Deoxyribonuclease I-like 3; DHP 2; DHP2; DNAS1L3; DNase gamma; DNase I homolog protein 2; DNase I homolog protein DHP2; DNase I like 3; DNase I-like 3; DNASE1L3; DNSL3_HUMAN; Liver and spleen DNase; LS DNase; LS-DNase; LSD; SLEB 16; SLEB16
• Gene ID: 1776
• mRNA Refseq: NM_001256560.2
• Protein Refseq: NP_001243489.1
• MIM: 602244
• UniProt ID: Q13609

Reviews

01/30/2023

The proteins accepted were of very high purity and were put into experiments with satisfactory results.

04/01/2022

Fast and timely shipment helped me to get satisfactory experimental results in short time.

07/21/2021

Excellent after-sales service, solving many experimental problems.

Q&As

What biological processes are DNASE1L3 involved in?

DNASE1L3 plays an important role in cell apoptosis and cell necrosis.

What does DNASE1L3 have to do with NET?

DNASE1L3 interacts with NET.

What diseases has DNASE1L3 been linked to studies of?

DNASE1L3 has been associated with the study of diseases such as hepatocellular carcinoma, gastric adenocarcinoma and colon cancer.

How does DNASE1L3 affect the development of hepatocellular carcinoma?

DNASE1L3 inhibits hepatocellular carcinoma progression by delaying cell cycle progression and attenuating glycolysis in hepatocellular carcinoma.