Recombinant Human DNMT3B protein, GST-tagged

• Cat.No.: DNMT3B-3712H
• Product Overview: Recombinant Human DNMT3B protein(1-100 aa), fused to GST tag, was expressed in E. coli.

Specification

• Source: E. coli
• Species: Human
• Tag: GST
• Form: The purified protein was Lyophilized from sterile PBS (58mM Na2HPO4,17mM NaH2PO4, 68mM NaCl, pH8.). 5 % trehalose and 5 % mannitol are added as protectant before lyophilization. The elution buffer contain 100mM GSH.
• Protein length: 1-100 aa
• AA Sequence: MKGDTRHLNGEEDAGGREDSILVNG ACSDQSSDSPPILEAIRTPEIRGRR SSSRLSKREVSSLLSYTQDLTGDGD GEDGDGSDTPVMPKLFRETRTRSES
• Purity: 95%, by SDS-PAGE with Coomassie Brilliant Blue staining.
• Storage: Short-term storage: Store at 2-8°C for (1-2 weeks).
  Long-term storage: Aliquot and store at -20°C to -80°C for up to 3 months, buffer containing 50% glycerol is recommended for reconstitution. Avoid repeat freeze-thaw cycles.
• Reconstitution: Reconstitute at 0.25 µg/μl in 200 μl sterile water for short-term storage.
  Reconstitution with 200 μl 50% glycerol solution is recommended for longer term storage.

Gene Information

• Gene Name: DNMT3B DNA (cytosine-5-)-methyltransferase 3 beta [ Homo sapiens ]
• Official Symbol: DNMT3B
• Synonyms: DNMT3B; DNA (cytosine-5-)-methyltransferase 3 beta; DNA (cytosine-5)-methyltransferase 3B; DNA MTase HsaIIIB; DNA methyltransferase HsaIIIB; ICF; ICF1; M.HsaIIIB
• Gene ID: 1789
• mRNA Refseq: NM_001207055
• Protein Refseq: NP_001193984
• MIM: 602900
• UniProt ID: Q9UBC3

Reviews

11/09/2020

The DNMT3B protein is of exceptional quality and has proven to be an ideal choice to meet my experimental requirements.

09/10/2020

With its consistent and outstanding results, the DNMT3B protein is an indispensable tool that I highly recommend for researchers in need of reliable and precise protein analysis.

04/23/2019

Its purity and reliability have consistently resulted in successful outcomes in my research projects, providing me with accurate and reproducible data.

Q&As

What are the potential side effects of targeting DNMT3B for therapeutic purposes?

Targeting DNMT3B for therapeutic purposes may lead to off-target effects and unintended changes in DNA methylation, potentially causing adverse effects on normal cellular function and gene expression.

Are there any genetic disorders associated with mutations in the DNMT3B gene?

Yes, mutations in the DNMT3B gene are linked to a rare genetic disorder called ICF (Immunodeficiency, Centromeric instability, Facial anomalies) syndrome, characterized by immune system deficiencies, facial abnormalities, and unstable centromeric regions in chromosomes.

Can DNMT3B protein be used as a potential biomarker for cancer diagnosis or prognosis?

Research has shown that alterations in DNMT3B expression or activity can be associated with various types of cancer, making it a potential biomarker for cancer diagnosis, prognosis, and treatment response.

How does DNMT3B protein contribute to epigenetic regulation in cells?

DNMT3B plays a crucial role in establishing de novo DNA methylation patterns during embryonic development and cell differentiation, contributing to the epigenetic regulation of gene expression.

What are the therapeutic implications of targeting DNMT3B in cancer treatment?

Inhibition of DNMT3B activity through targeted therapy or epigenetic modifying agents has been explored as a potential treatment strategy to reverse abnormal DNA methylation patterns and restore normal gene expression in cancer cells.