Recombinant Human DOCK8 Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: DOCK8-3734H
• Product Overview: DOCK8 MS Standard C13 and N15-labeled recombinant protein (NP_982272) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: This gene encodes a member of the DOCK180 family of guanine nucleotide exchange factors. Guanine nucleotide exchange factors interact with Rho GTPases and are components of intracellular signaling networks. Mutations in this gene result in the autosomal recessive form of the hyper-IgE syndrome. Alternatively spliced transcript variants encoding different isoforms have been described.
• Source: HEK293
• Species: Human
• Tag: Myc/DDK
• Molecular Mass: 238.3 kDa
• AA Sequence: MTHLNSLDVQLAQELGDFTDDDLDV VFTPKECRTLQPSLPEEGVELDPHV RDCVQTYIREWLIVNRKNQGSPEIC GFKKTGSRKDFHKTLPKQTFESETL ECSEPAAQAGPRHLNVLCDVSGKGP VTACDFDLRSLQPDKRLENLLQQVS AEDFEKQNEEARRTNRQAELFALYP SVDEEDAVEIRPVPECPKEHLGNRI LVKLLTLKFEIEIEPLFASIALYDV KERKKISENFHCDLNSDQFKGFLRA HTPSVAASSQARSAVFSVTYPSSDI YLVVKIEKVLQQGEIGDCAEPYTVI KESDGGKSKEKIEKLKLQAESFCQR LGKYRMPFAWAPISLSSFFSVSTLE REVTDVDSVVGRSSVGERRTLAQSR RLSERALSLEENGVGSNFKTSTLSV SIFFKQEGDRLSDEDLFKFLADYKR SSSLQRRVKSIPGLLRLEISTAPEI INCCLTPEMLPVKPFPENRTRPHKE ILEFPTREVYVPHTVYRNLLYVYPQ RLNFVNKLASARNITIKIQFMCGED ASNAMPVIFGKSSGPEFLQEVYTAV TYHNKSPDFYEEVKIKLPAKLTVNH HLLFTFYHISCQQKQGASVETLLGY SWLPILLNERLQTGSYCLPVALEKL PPNYSMHSAEKVPLQNPPIKWAEGH KGVFNIEVQAVSSVHTQDNHLEKFF TLCHSLESQVTFPIRVLDQKISEMA LEHELKLSIICLNSSRLEPLVLFLH LVLDKLFQLSVQPMVIAGQTANFSQ FAFESVVAIANSLHNSKDLSKDQHG RNCLLASYVHYVFRLPEVQRDVPKS DAPTALLDPRSYHTYGRTSAAAVSS KLLQARVMSSSNPDLAGTHSAADEE VKNIMSSKIADRNCSRMSYYCSGSS DAPSSPAAPRPASKKHFHEELALQM VVSTGMVRETVFKYAWFFFELLVKS MAQHVHNMDKRDSFRRTRFSDRFMD DITTIVNVVTSEIAALLVKPQKENE QAEKMNISLAFFLYDLLSLMDRGFV FNLIRHYCSQLSAKLSNLPTLISMR LEFLRILCSHEHYLNLNLFFMNADT APTSPCPSISSQNSSSCSSFQDQKI ASMFDLTSEYRQQHFLTGLLFTELA AALDAEGEGISKVQRKAVSAIHSLL SSHDLDPRCVKPEVKVKIAALYLPL VGIILDALPQLCDFTVADTRRYRTS GSDEEQEGAGAINQNVALAIAGNNF NLKTSGIVLSSLPYKQYNMLNADTT RNLMICFLWIMKNADQSLIRKWIAD LPSTQLNRILDLLFICVLCFEYKGK QSSDKVSTQVLQKSRDVKARLEEAL LRGEGARGEMMRRRAPGNDRFPGLN ENLRWKKEQTHWRQANEKLDKTKAE LDQEALISGNLATEAHLIILDMQEN IIQASSALDCKDSLLGGVLRVLVNS LNCDQSTTYLTHCFATLRALIAKFG DLLFEEEVEQCFDLCHQVLHHCSSS MDVTRSQACATLYLLMRFSFGATSN FARVKMQVTMSLASLVGRAPDFNEE HLRRSLRTILAYSEEDTAMQMTPFP TQVEELLCNLNSILYDTVKMREFQE DPEMLMDLMYRIAKSYQASPDLRLT WLQNMAEKHTKKKCYTEAAMCLVHA AALVAEYLSMLEDHSYLPVGSVSFQ NISSNVLEESVVSEDTLSPDEDGVC AGQYFTESGLVGLLEQAAELFSTGG LYETVNEVYKLVIPILEAHREFRKL TLTHSKLQRAFDSIVNKDHKRMFGT YFRVGFFGSKFGDLDEQEFVYKEPA ITKLPEISHRLEAFYGQCFGAEFVE VIKDSTPVDKTKLDPNKAYIQITFV EPYFDEYEMKDRVTYFEKNFNLRRF MYTTPFTLEGRPRGELHEQYRRNTV LTTMHAFPFIKTRISVIQKEEFVLT PIEVAIEDMKKKTLQLAVAINQEPP DAKMLQMVLQGSVGATVNQGPLEVA QVFLAEIPADPKLYRHHNKLRLCFK EFIMRCGEAVEKNKRLITADQREYQ QELKKNYNKLKENLRPMIERKIPEL YKPIFRVESQKRDSFHRSSFRKCET QLSQGSTRTRPLEQKLISEEDLAAN DILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: DOCK8 dedicator of cytokinesis 8 [ Homo sapiens (human) ]
• Official Symbol: DOCK8
• Synonyms: DOCK8; dedicator of cytokinesis 8; MRD2; ZIR8; HEL-205; dedicator of cytokinesis protein 8; 1200017A24Rik; epididymis luminal protein 205
• Gene ID: 81704
• mRNA Refseq: NM_203447
• Protein Refseq: NP_982272
• MIM: 611432
• UniProt ID: Q8NF50