Recombinant Human DPH2 Protein (1-489 aa), His-SUMO-tagged

• Cat.No.: DPH2-1111H
• Product Overview: Recombinant Human DPH2 Protein (1-489 aa) is produced by E. coli expression system. This protein is fused with a 6xHis-SUMO tag at the N-terminal. Research Area: Cell Biology. Protein Description: Full Length.

Specification

• Description: Required for the first step in the synthesis of diphthamide, a post-translational modification of histidine which occurs in translation elongation factor 2 (EEF2).
• Source: E. coli
• Species: Human
• Tag: His-SUMO
• Form: Tris-based buffer, 50% glycerol
• Molecular Mass: 68.1 kDa
• Protein length: 1-489 aa
• AA Sequence: MESMFSSPAEAALQRETGVPGLLTP LPDLDGVYELERVAGFVRDLGCERV ALQFPDQLLGDAVAVAARLEETTGS KMFILGDTAYGSCCVDVLGAEQAGA QALIHFGPACLSPPARPLPVAFVLR QRSVALELCVKAFEAQNPDPKAPVV LLSEPACAHALEALATLLRPRYLDL LVSSPAFPQPVGSLSPEPMPLERFG RRFPLAPGRRLEEYGAFYVGGSKAS PDPDLDPDLSRLLLGWAPGQPFSSC CPDTGKTQDEGARAGRLRARRRYLV ERARDARVVGLLAGTLGVAQHREAL AHLRNLTQAAGKRSYVLALGRPTPA KLANFPEVDVFVLLACPLGALAPQL SGSFFQPILAPCELEAACNPAWPPP GLAPHLTHYADLLPGSPFHVALPPP ESELWETPDVSLITGDLRPPPAWKS SNDHGSLALTPRPQLELAESSPAAS FLSSRSWQGLEPRLGQTPVTEAVSG RRGIAIAYEDEGSG
• Purity: > 90% as determined by SDS-PAGE.
• Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 centigrade for up to one week.
• Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 centigrade/-80 centigrade. The shelf life of lyophilized form is 12 months at -20 centigrade/-80 centigrade.
• Concentration: A hardcopy of COA with concentration instruction is sent along with the products.

Gene Information

• Gene Name: DPH2 DPH2 homolog (S. cerevisiae) [ Homo sapiens ]
• Official Symbol: DPH2
• Synonyms: DPH2; DPH2-like 2; DPH2L2
• Gene ID: 1802
• mRNA Refseq: NM_001039589
• Protein Refseq: NP_001034678
• MIM: 603456
• UniProt ID: Q9BQC3