Recombinant Human DROSHA Lysate

• Cat.No.: DROSHA-424HCL
• Product Overview: Recombinant Human DROSHA protein (as WB positive control) was fused at Myc/DDK-tagged at the C-terminus and overexpressed in human 293 cells (HEK293).

Specification

• Description: This gene encodes a ribonuclease (RNase) III double-stranded RNA-specific ribonuclease and subunit of the microprocessor protein complex, which catalyzes the initial processing step of microRNA (miRNA) synthesis. The encoded protein cleaves the stem loop structure from the primary microRNA (pri-miRNA) in the nucleus, yielding the precursor miRNA (pre-miRNA), which is then exported to the cytoplasm for further processing. In a human cell line lacking a functional copy of this gene, canonical miRNA synthesis is reduced. Somatic mutations in this gene have been observed in human patients with kidney cancer.
• Source: HEK293
• Species: Human
• Tag: Myc/DDK
• Form: 1 vial of 20 ug lyophilized gene specific transient over-expression cell lysate.
• Molecular Mass: 159.1 kDa
• Applications: WB positive control
• Storage: The lysate can be shipped at ambient temperature. Upon receiving, store the sample at -20 centigrade. Lysate samples can be reconstituted with SDS Sample Buffer. Avoid repeated freeze-thaw cycles after reconstitution. Lysate samples are stable for 12 months from date of receipt when stored at -20 centigrade.

Gene Information

• Gene Name: DROSHA
• Official Symbol: DROSHA
• Synonyms: RN3; ETOHI2; RNASEN; RANSE3L; RNASE3L; HSA242976
• Gene ID: 29102
• mRNA Refseq: NM_013235.5
• Protein Refseq: NP_037367.3
• MIM: 608828
• UniProt ID: Q9NRR4