Recombinant Human DUT Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: DUT-3772H
• Product Overview: DUT MS Standard C13 and N15-labeled recombinant protein (NP_001939) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: This gene encodes an essential enzyme of nucleotide metabolism. The encoded protein forms a ubiquitous, homotetrameric enzyme that hydrolyzes dUTP to dUMP and pyrophosphate. This reaction serves two cellular purposes: providing a precursor (dUMP) for the synthesis of thymine nucleotides needed for DNA replication, and limiting intracellular pools of dUTP. Elevated levels of dUTP lead to increased incorporation of uracil into DNA, which induces extensive excision repair mediated by uracil glycosylase. This repair process, resulting in the removal and reincorporation of dUTP, is self-defeating and leads to DNA fragmentation and cell death. Alternative splicing of this gene leads to different isoforms that localize to either the mitochondrion or nucleus. A related pseudogene is located on chromosome 19.
• Source: HEK293
• Species: Human
• Tag: Myc/DDK
• Molecular Mass: 17.6 kDa
• AA Sequence: MPCSEETPAISPSKRARPAEVGGMQ LRFARLSEHATAPTRGSARAAGYDL YSAYDYTIPPMEKAVVKTDIQIALP SGCYGRVAPRSGLAAKHFIDVGAGV IDEDYRGNVGVVLFNFGKEKFEVKK GDRIAQLICERIFYPEIEEVQALDD TERGSGGFGSTGKNTRTRPLEQKLI SEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: DUT deoxyuridine triphosphatase [ Homo sapiens (human) ]
• Official Symbol: DUT
• Synonyms: DUT; deoxyuridine triphosphatase; dUTP pyrophosphatase; deoxyuridine 5-triphosphate nucleotidohydrolase, mitochondrial; dUTPase; dUTP nucleotidohydrolase; FLJ20622
• Gene ID: 1854
• mRNA Refseq: NM_001948
• Protein Refseq: NP_001939
• MIM: 601266
• UniProt ID: P33316

Reviews

04/01/2021

The DUT protein demonstrates significant relevance and potential in various trial applications, primarily focusing on inflammation, angiogenesis, and tissue remodeling.

04/21/2020

Another advantage of the DUT protein is its reliability and consistency, ensuring robust and reproducible results.

05/12/2017

Whether it is regarding experimental design, optimization of protocols, or troubleshooting issues, their expertise and prompt customer service help researchers overcome challenges efficiently.

Q&As

Can the DUT protein be used as a biomarker in cancer diagnosis?

Yes, alterations in the expression levels of the DUT protein can serve as a biomarker for certain cancers, aiding in early diagnosis and prognosis.

How does the DUT protein contribute to the understanding of neurodegenerative diseases?

Research suggests a link between the DUT protein and neurodegenerative diseases, where its dysfunction may impact DNA repair processes in neurons, potentially contributing to disease progression.

Are there any specific cancer types where the DUT protein is particularly relevant?

Research suggests that the DUT protein is particularly relevant in colorectal cancer, where its abnormal expression may impact DNA replication and repair mechanisms.

How is the DUT protein clinically significant in cancer research?

The DUT protein is implicated in cancer research as its dysregulation can lead to an imbalance in nucleotide pools, potentially contributing to genomic instability, a common feature in cancer cells.

How does the DUT protein contribute to the development of targeted therapies in cancer treatment?

Understanding the role of the DUT protein helps in developing targeted therapies that aim to modulate its activity, potentially disrupting cancer cell proliferation.