Recombinant Human EED protein(Met1-Arg441), His&GST-tagged

• Cat.No.: EED-6921H
• Product Overview: Recombinant Human EED (O75530-1) (Met 1-Arg 441) was expressed in Insect Cells, fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Specification

• Source: Insect Cells
• Species: Human
• Tag: N-His&GST
• Protein length: Met1-Arg441
• Form: Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 8.0, 2mM GSH, 10% gly Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Molecular Mass: The recombinant human EED/GST chimera consists of 678 amino acids and has a calculated molecular mass of 78 kDa. It migrates as an approximately 75-85 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 93 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
• Publication:
  Polycomb repressive complex 2 binds and stabilizes NANOG to suppress differentiationrelated genes to promote self-renewal (2023)

Gene Information

• Gene Name: EED embryonic ectoderm development [ Homo sapiens ]
• Official Symbol: EED
• Synonyms: EED; embryonic ectoderm development; polycomb protein EED; HEED; WAIT 1; WD protein associating with integrin cytoplasmic tails 1; WAIT1
• Gene ID: 8726
• mRNA Refseq: NM_003797
• Protein Refseq: NP_003788
• MIM: 605984
• UniProt ID: O75530

Reviews

12/25/2021

Precise protein quantification, ensures data accuracy in our experiments.

07/03/2020

Dependable peptide synthesis, accelerates our projects effectively.

03/02/2020

Timely antibody validation, a valuable asset for our lab.

Q&As

What is the impact of EED dysregulation on the development of cancer, especially in terms of epigenetic alterations?

Dysregulation of EED can lead to cancer, largely through epigenetic alterations and disrupted gene regulation.

How does EED contribute to the maintenance of pluripotency and self-renewal in stem cells?

EED helps maintain stem cell pluripotency and self-renewal capabilities, crucial for embryonic development.

How does EED interact with the Polycomb repressive complex 2 (PRC2) in chromatin remodeling?

EED works with PRC2 in chromatin remodeling, influencing gene silencing and cellular identity.

How do alterations in EED expression or function affect developmental disorders and congenital anomalies?

Changes in EED function impact developmental processes, potentially leading to congenital anomalies and disorders.

How does EED regulate epigenetic modifications, particularly histone methylation, in gene expression?

EED regulates gene expression by modulating histone methylation, affecting epigenetic landscapes.

What role does EED play in the development and differentiation of embryonic stem cells?

EED is essential in embryonic stem cell development, guiding differentiation processes.

What are the mechanisms by which EED influences cellular aging and senescence?

EED affects cellular aging and senescence, playing a role in age-related epigenetic changes.