Active Recombinant Human EGFRvIII Protein, His & Avi-tagged, Biotinylated
Cat.No. : | EGFR-341H |
Product Overview : | Recombinant Human EGFRvIII Protein, His & Avi-tagged, Biotinylated is expressed from human 293 cells (HEK293). It contains AA Leu 25 - Ser 645 (30-297: Val Cys Gln...Cys Pro Arg → Gly) (Accession # NP_005219.2). Predicted N-terminus: Leu 25. This protein carries an Avi tag at the C-terminus, followed by a polyhistidine tag. The protein has a calculated MW of 41.3 kDa. As a result of glycosylation, the protein migrates as 60-70 kDa under reducing (R) condition, and 60-70 kDa under non-reducing (NR) condition (SDS-PAGE). The biotin to protein ratio is 0.5-1 as determined by the HABA assay. |
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Source : | HEK293 |
Species : | Human |
Tag : | His & Avi |
Form : | Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization. |
Bio-activity : | Immobilized CetuxiMabat 2 μg/mL (100 μL/well) can bind Recombinant Human EGFRvIII Protein, His & Avi-tagged, Biotinylated with a linear range of 0.4-25 ng/mL (QC tested). |
Molecular Mass : | 41.3 kDa |
Protein length : | Leu 25 - Ser 645 |
AA Sequence : | LEEKKGNYVVTDHGSCVRACGADSY EMEEDGVRKCKKCEGPCRKVCNGIG IGEFKDSLSINATNIKHFKNCTSIS GDLHILPVAFRGDSFTHTPPLDPQE LDILKTVKEITGFLLIQAWPENRTD LHAFENLEIIRGRTKQHGQFSLAVV SLNITSLGLRSLKEISDGDVIISGN KNLCYANTINWKKLFGTSGQKTKII SNRGENSCKATGQVCHALCSPEGCW GPEPRDCVSCRNVSRGRECVDKCNL LEGEPREFVENSECIQCHPECLPQA MNITCTGRGPDNCIQCAHYIDGPHC VKTCPAGVMGENNTLVWKYADAGHV CHLCHPNCTYGCTGPGLEGCPTNGP KIPSGLNDIFEAQKIEWHEHHHHHH |
Endotoxin : | Less than 1.0 EU per μg by the LAL method. |
Purity : | >95% as determined by SDS-PAGE. |
Notes : | Please avoid repeated freeze-thaw cycles. |
Stability : | No activity loss is observed after storage at: 4-8 centigrade for 12 months in lyophilized state; -70 centigrade for 3 months under sterile conditions after reconstitution. |
Storage : | For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower. |
Reconstitution : | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4 centigrade before opening to recover the entire contents. |
Gene Name : | EGFR epidermal growth factor receptor [ Homo sapiens ] |
Official Symbol : | EGFR |
Synonyms : | EGFR; epidermal growth factor receptor; ERBB1; ERBB; HER1; mENA; PIG61; |
Gene ID : | 1956 |
mRNA Refseq : | NM_005228 |
Protein Refseq : | NP_005219 |
MIM : | 131550 |
UniProt ID : | P00533 |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (6)
Ask a questionIt is associated with biological processes in human embryonic development and tissue regeneration.
EGFR inhibits adverse drug reactions such as rash, inflammation, and diarrhea during drug treatment.
The role of EGFR in tumor treatment is to act as a molecular marker and can be used as a prognostic indicator and monitor tumor progression; at the same time, EGFR mutations can also be targeted for targeted therapy.
EGFR gene mutation detection generally uses PCR, fluorescence in situ hybridization, and direct sequencing.
The combination of EGFR inhibitors with chemotherapy drugs, radiotherapy, immunotherapy, etc. can increase the efficacy of treatment and reduce the occurrence of adverse reactions.
EGFR mutations can have a significant impact on the efficacy of targeted therapies, and patients with mutations have higher response rates.
Customer Reviews (3)
Write a reviewThe powerful biological activity of EGFR combined with its high purity makes the experimental results highly reproducible, which is of great significance in the field of scientific research.
Due to the short half-life and high clearance characteristics of EGFR, they can better adapt to the needs of clinical treatment and provide more treatment options for patients.
The production process of EGFR uses advanced biotechnology, which makes the production process more efficient and environmentally friendly, and reduces the consumption of human and material resources.
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