Recombinant Human EP300 Protein

• Cat.No.: EP300-2965H
• Product Overview: Recombinant human EP300 protein without tag was expressed in E. coli.

Specification

• Description: Functions as histone acetyltransferase and regulates transcription via chromatin remodeling. Acetylates all four core histones in nucleosomes. Histone acetylation gives an epigenetic tag for transcriptional activation. Mediates cAMP-gene regulation by binding specifically to phosphorylated CREB protein. Mediates acetylation of histone H3 at' Lys-122' (H3K122ac), a modification that localizes at the surface of the histone octamer and stimulates transcription, possibly by promoting nucleosome instability. Mediates acetylation of histone H3 at' Lys-27' (H3K27ac). Also functions as acetyltransferase for non-histone targets, such as ALX1, HDAC1, PRMT1 or SIRT2. Acetylates' Lys-131' of ALX1 and acts as its coactivator.
• Source: E. coli
• Species: Human
• Tag: Tag Free
• Purity: >90 % as determined by SDS-PAGE.
• Notes: For research use only.
• Storage: Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8 centigrade for one week. Store at -20 to -80 centigrade for twelve months from the date of receipt.
• Storage Buffer: Supplied as solution form in PBS pH 7.5 or lyophilized from PBS pH 7.5.
• Reconstitution: Reconstitute in sterile water for a stock solution.
• Shipping: In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.

Gene Information

• Gene Name: EP300 E1A binding protein p300 [ Homo sapiens (human) ]
• Official Symbol: EP300
• Synonyms: EP300; E1A binding protein p300; histone acetyltransferase p300; KAT3B; p300; p300 HAT; E1A-binding protein, 300kD; E1A-associated protein p300; RSTS2
• Gene ID: 2033
• mRNA Refseq: NM_001429
• Protein Refseq: NP_001420
• MIM: 602700
• UniProt ID: Q09472

Reviews

09/22/2022

It has consistently demonstrated outstanding results, consistently producing clear and sharp bands, allowing for accurate and reliable protein detection and quantification.

03/09/2021

Its stability and purity make it an ideal candidate for studying protein structures at a high resolution, providing crucial insights into the organization and function of the EP300 protein.

07/07/2016

I highly recommend the EP300 protein for its exceptional performance in Western blot analysis.

Q&As

How does EP300 impact developmental abnormalities?

EP300 is crucial for embryonic development, and mutations in the EP300 gene can lead to developmental abnormalities. Understanding its role is essential for potential interventions.

How does EP300 contribute to neurodegenerative disorders?

EP300 is involved in neuronal function and survival. Dysregulation of EP300 has been observed in neurodegenerative diseases like Alzheimer's and Parkinson's.

Are there experimental treatments targeting EP300 in neurodegenerative disorders?

Research is underway to explore drugs that modulate EP300 activity for potential therapeutic interventions in neurodegenerative disorders, but clinical applications are still in the early stages.

Are there any ongoing clinical trials targeting EP300 in cancer treatment?

Yes, there are clinical trials exploring the therapeutic potential of targeting EP300 in various cancer types, focusing on its role in controlling cell proliferation and survival.

Are there drugs targeting EP300 in cardiovascular diseases?

While there are no specific drugs targeting EP300 approved for cardiovascular diseases, ongoing research aims to identify compounds that can modulate its activity for therapeutic purposes.