Recombinant Human EPHB4 protein(Met1-Ala539)

• Cat.No.: EPHB4-697H
• Product Overview: Recombinant Human EphB4 (NP_004435.3) (Met 1-Ala 539) with quinary-aa peptide (DDDDK) at the C-terminus was expressed and purified in HEK293.

Specification

• Source: HEK293
• Species: Human
• Tag: N/A
• Protein length: Met1-Ala539
• Form: Lyophilized from sterile 100mM NaCl, 50mM Tris, pH 7.5. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its binding ability in a functional ELISA. Immobilized human EphB4 at 2 μg/ml (100 μl/well) can bind human EphrinB2 with a linear range of 1-125 ng/ml.
• Molecular Mass: The recombinant human EphB4 is a monomeric protein consisting of 530 amino acids and has a calculated molecular mass of 57.7 kDa. As a result of glycosylation, rhEphB4 migrates as an approximately 65-70 kDa protein in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: EPHB4 EPH receptor B4 [ Homo sapiens ]
• Official Symbol: EPHB4
• Synonyms: EPHB4; EPH receptor B4; EphB4 , HTK; ephrin type-B receptor 4; Tyro11; ephrin receptor EphB4; soluble EPHB4 variant 1; soluble EPHB4 variant 2; soluble EPHB4 variant 3; hepatoma transmembrane kinase; tyrosine-protein kinase TYRO11; tyrosine-protein kinase receptor HTK; HTK; MYK1; TYRO11
• Gene ID: 2050
• mRNA Refseq: NM_004444
• Protein Refseq: NP_004435
• MIM: 600011
• UniProt ID: P54760