Recombinant Human EPHX2 Protein (1-555 aa), His-SUMO-tagged

• Cat.No.: EPHX2-483H
• Product Overview: Recombinant Human EPHX2 Protein (1-555 aa) is produced by E. coli expression system. This protein is fused with a 6xHis-SUMO tag at the N-terminal. Research Area: Cancer. Protein Description: Full Length.

Specification

• Description: Bifunctional enzyme. The C-terminal domain has epoxide hydrolase activity and acts on epoxides (alkene oxides, oxiranes) and arene oxides. Plays a role in xenobiotic metabolism by degrading potentially toxic epoxides. Also determines steady-state levels of physiological mediators. The N-terminal domain has lipid phosphatase activity, with the highest activity towards threo-9,10-phosphonooxy-hydroxy-octadecanoic acid, followed by erythro-9,10-phosphonooxy-hydroxy-octadecanoic acid, 12-phosphonooxy-octadec-9Z-enoic acid, 12-phosphonooxy-octadec-9E-enoic acid, and p-nitrophenyl phospate.
• Source: E. coli
• Species: Human
• Tag: His-SUMO
• Form: Tris-based buffer, 50% glycerol
• Molecular Mass: 78.6 kDa
• Protein length: 1-555 aa
• AA Sequence: MTLRAAVFDLDGVLALPAVFGVLGR TEEALALPRGLLNDAFQKGGPEGAT TRLMKGEITLSQWIPLMEENCRKCS ETAKVCLPKNFSIKEIFDKAISARK INRPMLQAALMLRKKGFTTAILTNT WLDDRAERDGLAQLMCELKMHFDFL IESCQVGMVKPEPQIYKFLLDTLKA SPSEVVFLDDIGANLKPARDLGMVT ILVQDTDTALKELEKVTGIQLLNTP APLPTSCNPSDMSHGYVTVKPRVRL HFVELGSGPAVCLCHGFPESWYSWR YQIPALAQAGYRVLAMDMKGYGESS APPEIEEYCMEVLCKEMVTFLDKLG LSQAVFIGHDWGGMLVWYMALFYPE RVRAVASLNTPFIPANPNMSPLESI KANPVFDYQLYFQEPGVAEAELEQN LSRTFKSLFRASDESVLSMHKVCEA GGLFVNSPEEPSLSRMVTEEEIQFY VQQFKKSGFRGPLNWYRNMERNWKW ACKSLGRKILIPALMVTAEKDFVLV PQMSQHMEDWIPHLKRGHIEDCGHW TQMDKPTEVNQILIKWLDSDARNPP VVSKM
• Purity: > 90% as determined by SDS-PAGE.
• Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 centigrade for up to one week.
• Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 centigrade/-80 centigrade. The shelf life of lyophilized form is 12 months at -20 centigrade/-80 centigrade.
• Concentration: A hardcopy of COA with concentration instruction is sent along with the products.

Gene Information

• Gene Name: EPHX2 epoxide hydrolase 2, cytoplasmic [ Homo sapiens ]
• Official Symbol: EPHX2
• Synonyms: EPHX2; CEH; SEH
• Gene ID: 2053
• mRNA Refseq: NM_001256482
• Protein Refseq: NP_001243411
• MIM: 132811
• UniProt ID: P34913