Active Recombinant Human ERBB2 Protein, His-Avi-tagged, Biotinylated
Cat.No. : | ERBB2-183H |
Product Overview : | Biotinylated Human Her2, His Tag, Avi Tag is expressed from HEK293 cells. It contains AA Thr 23 - Thr 652 (Accession # P04626-1). This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag. |
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Source : | HEK293 |
Species : | Human |
Tag : | His-Avi |
Predicted N Terminal : | Thr 23 |
Form : | Lyophilized from 0.22 um filtered solution in PBS, pH7.4, 10% trehalose. |
Bio-activity : | Immobilized Trastuzumab at 3 ug/mL (100 uL/well) can bind Biotinylated Human Her2, His Tag, Avi Tag with a linear range of 0.2-6 ug/mL. |
Molecular Mass : | The protein has a calculated MW of 73.0 kDa. The protein migrates as 85-95 KDa under reducing condition (SDS-PAGE) due to glycosylation. |
Endotoxin : | < 1.0 EU per μg of the protein as determined by the LAL method. |
Purity : | >90% as determined by SDS-PAGE. |
Characteristic : | Biotinylation of this product is performed using Avitag technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin. |
Stability : | Samples are stable for up to twelve months from date of receipt at -70ºC. |
Storage : | For long term storage, the product should be stored at lyophilized state at -20ºC or lower. Please avoid repeated freeze-thaw cycles. No activity loss is observed after storage at: 4-8ºC for 12 months in lyophilized state; -70ºC for 3 months under sterile conditions after reconstitution. |
Reconstitution : | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents. |
Gene Name : | ERBB2 v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian) [ Homo sapiens ] |
Official Symbol : | ERBB2 |
Synonyms : | ERBB2; v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian); NGL, v erb b2 avian erythroblastic leukemia viral oncogene homolog 2 (neuro/glioblastoma derived oncogene homolog); receptor tyrosine-protein kinase erbB-2; CD340; HER 2; HER2; NEU; herstatin; p185erbB2; proto-oncogene Neu; c-erb B2/neu protein; proto-oncogene c-ErbB-2; metastatic lymph node gene 19 protein; tyrosine kinase-type cell surface receptor HER2; neuroblastoma/glioblastoma derived oncogene homolog; v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2 (neuro/glioblastoma derived oncogene homolog); NGL; TKR1; HER-2; MLN 19; HER-2/neu; |
Gene ID : | 2064 |
mRNA Refseq : | NM_001005862 |
Protein Refseq : | NP_001005862 |
MIM : | 164870 |
UniProt ID : | P04626 |
Products Types
◆ Recombinant Protein | ||
ERBB2-046H | Recombinant Human ERBB2 Protein, MYC/DDK-tagged, C13 and N15-labeled | +Inquiry |
ERBB2-1062H | Recombinant Human ERBB2 protein(Met1-Thr652), Biotinylated | +Inquiry |
ERBB2-285H | Recombinant Human ERBB2 Protein, Fc-tagged | +Inquiry |
ERBB2-2118H | Recombinant Human ERBB2 Protein, MYC/DDK-tagged | +Inquiry |
ERBB2-283H | Recombinant Human ERBB2 Protein, Fc-tagged | +Inquiry |
◆ Lysates | ||
ERBB2-1727MCL | Recombinant Mouse ERBB2 cell lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (8)
Ask a question1.Prepare the streptavidin-coated plate by washing it with PBS (phosphate-buffered saline) or another suitable buffer to remove any impurities. 2.Dilute the Avi-tagged protein in the desired buffer at the desired concentration. It is important to use a buffer that is compatible with the protein and does not interfere with its activity or stability. 3.Add the diluted protein to the streptavidin-coated plate and incubate it for a suitable amount of time at room temperature or 4°C. The exact conditions will depend on the specific protein and the desired level of immobilization. 4.Wash the plate with a suitable buffer to remove any unbound protein. 5.Block the remaining binding sites on the plate with a suitable blocking agent such as BSA (bovine serum albumin) or casein. 6.Wash the plate again to remove any unbound blocking agent. 7.Use the immobilized protein for further experiments such as ELISA, Western blotting, or other assays.
ERBB2 is a receptor tyrosine kinase involved in the regulation of cell growth and differentiation.
Therapeutic agents targeting ERBB2 include trastuzumab and lapatinib.
ERBB2 is commonly overexpressed in breast, ovarian, and stomach cancers.
ERBB2 overexpression is often associated with aggressive disease and poor prognosis.
ERBB2 levels can be assessed using immunohistochemistry and fluorescence in situ hybridization (FISH) in tissue samples.
It forms heterodimers with other ERBB receptors, activating pathways that promote cell growth and survival.
Amplification of the ERBB2 gene leads to its overexpression in cancer cells.
Customer Reviews (3)
Write a reviewWorked as expected, satisfied.
Reliable in protein assays.
Quick and reliable shipping.
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