Recombinant Human ETF1 Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: ETF1-3650H
• Product Overview: ETF1 MS Standard C13 and N15-labeled recombinant protein (NP_004721) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: This gene encodes a class-1 polypeptide chain release factor. The encoded protein plays an essential role in directing termination of mRNA translation from the termination codons UAA, UAG and UGA. This protein is a component of the SURF complex which promotes degradation of prematurely terminated mRNAs via the mechanism of nonsense-mediated mRNA decay (NMD). Alternate splicing results in multiple transcript variants. Pseudogenes of this gene are found on chromosomes 6, 7, and X.
• Source: HEK293
• Species: Human
• Tag: Myc/DDK
• Molecular Mass: 48.9 kDa
• AA Sequence: MADDPSAADRNVEIWKIKKLIKSLE AARGNGTSMISLIIPPKDQISRVAK MLADEFGTASNIKSRVNRLSVLGAI TSVQQRLKLYNKVPPNGLVVYCGTI VTEEGKEKKVNIDFEPFKPINTSLY LCDNKFHTEALTALLSDDSKFGFIV IDGSGALFGTLQGNTREVLHKFTVD LPKKHGRGGQSALRFARLRMEKRHN YVRKVAETAVQLFISGDKVNVAGLV LAGSADFKTELSQSDMFDQRLQSKV LKLVDISYGGENGFNQAIELSTEVL SNVKFIQEKKLIGRYFDEISQDTGK YCFGVEDTLKALEMGAVEILIVYEN LDIMRYVLHCQGTEEEKILYLTPEQ EKDKSHFTDKETGQEHELIESMPLL EWFANNYKKFGATLEIVTDKSQEGS QFVKGFGGIGGILRYRVDFQGMEYQ GGDDEFFDLDDYTRTRPLEQKLISE EDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: ETF1 eukaryotic translation termination factor 1 [ Homo sapiens (human) ]
• Official Symbol: ETF1
• Synonyms: ETF1; eukaryotic translation termination factor 1; ERF, ERF1, SUP45L1; eukaryotic peptide chain release factor subunit 1; eRF1; polypeptide chain release factor 1; RF1; sup45 (yeast omnipotent suppressor 45) homolog like 1; TB3 1; protein Cl1; eukaryotic release factor 1; sup45 (yeast omnipotent suppressor 45) homolog-like 1; ERF; ERF1; TB3-1; D5S1995; SUP45L1; MGC111066
• Gene ID: 2107
• mRNA Refseq: NM_004730
• Protein Refseq: NP_004721
• MIM: 600285
• UniProt ID: P62495