Recombinant Human F12 protein, His-tagged

• Cat.No.: F12-12617H
• Product Overview: Recombinant Human F12 protein(316-615 aa), fused with His tag, was expressed in E.coli.

Specification

• Source: E.coli
• Species: Human
• Tag: His
• Form: The purified protein was Lyophilized from sterile PBS (58mM Na2HPO4,17mM NaH2PO4, 68mM NaCl, pH7.4). 5 % trehalose and 5 % mannitol are added as protectant before lyophilization. The elution buffer contain 300mM imidazole.
• Protein Length: 316-615 aa
• AA Sequence: MPAQPAPPKPQPTTRTPPQSQTPGA LPAKREQPPSLTRNGPLSCGQRLRK SLSSMTRVVGGLVALRGAHPYIAAL YWGHSFCAGSLIAPCWVLTAAHCLQ DRPAPEDLTVVLGQERRNHSCEPCQ TLAVRSYRLHEAFSPVSYQHDLALL RLQEDADGSCALLSPYVQPVCLPSG AARPSETTLCQVAGWGHQFEGAEEY ASFLQEAQVPFLSLERCSAPDVHGS SILPGMLCAGFLEGGTDACQGDSGG PLVCEDQAAERRLTLQGIISWGSGC GDRNKPGVYTDVAYYLAWIREHTVS
• Purity: 85%, by SDS-PAGE with Coomassie Brilliant Blue staining.
• Storage: Short-term storage: Store at 2-8°C for (1-2 weeks).
  Long-term storage: Aliquot and store at -20°C to -80°C for up to 3 months, buffer containing 50% glycerol is recommended for reconstitution. Avoid repeat freeze-thaw cycles.
• Reconstitution: Reconstitute at 0.25 µg/μl in 200 μl sterile water for short-term storage.
  Reconstitution with 200 μl 50% glycerol solution is recommended for longer term storage.

Gene Information

• Gene Name: F12 coagulation factor XII (Hageman factor) [ Homo sapiens ]
• Official Symbol: F12
• Synonyms: F12; coagulation factor XII (Hageman factor); coagulation factor XII; Hageman factor; beta-factor XIIa part 1; beta-factor XIIa part 2; coagulation factor XIIa heavy chain; coagulation factor XIIa light chain; HAF; HAE3; HAEX
• Gene ID: 2161
• mRNA Refseq: NM_000505
• Protein Refseq: NP_000496
• MIM: 610619
• UniProt ID: P00748