Recombinant Human FBL Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: FBL-2503H
• Product Overview: FBL MS Standard C13 and N15-labeled recombinant protein (NP_001427) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: This gene product is a component of a nucleolar small nuclear ribonucleoprotein (snRNP) particle thought to participate in the first step in processing preribosomal RNA. It is associated with the U3, U8, and U13 small nuclear RNAs and is located in the dense fibrillar component (DFC) of the nucleolus. The encoded protein contains an N-terminal repetitive domain that is rich in glycine and arginine residues, like fibrillarins in other species. Its central region resembles an RNA-binding domain and contains an RNP consensus sequence. Antisera from approximately 8% of humans with the autoimmune disease scleroderma recognize fibrillarin.
• Source: HEK293
• Species: Human
• Tag: Myc/DDK
• Molecular Mass: 33.6 kDa
• AA Sequence: MKPGFSPRGGGFGGRGGFGDRGGRG GRGGFGGGRGRGGGFRGRGRGGGGG GGGGGGGGRGGGGFHSGGNRGRGRG GKRGNQSGKNVMVEPHRHEGVFICR GKEDALVTKNLVPGESVYGEKRVSI SEGDDKIEYRAWNPFRSKLAAAILG GVDQIHIKPGAKVLYLGAASGTTVS HVSDIVGPDGLVYAVEFSHRSGRDL INLAKKRTNIIPVIEDARHPHKYRM LIAMVDVIFADVAQPDQTRIVALNA HTFLRNGGHFVISIKANCIDSTASA EAVFASEVKKMQQENMKPQEQLTLE PYERDHAVVVGVYRPPPKVKNTRTR PLEQKLISEEDLAANDILDYKDDDD KV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: FBL fibrillarin [ Homo sapiens (human) ]
• Official Symbol: FBL
• Synonyms: FBL; fibrillarin; rRNA 2-O-methyltransferase fibrillarin; FIB; FLRN; RNU3IP1; 34-kD nucleolar scleroderma antigen; 34 kDa nucleolar scleroderma antigen; RNA, U3 small nucleolar interacting protein 1
• Gene ID: 2091
• mRNA Refseq: NM_001436
• Protein Refseq: NP_001427
• MIM: 134795
• UniProt ID: P22087