| Description : |
The protein encoded by this gene removes 5' overhanging flaps in DNA repair and processes the 5' ends of Okazaki fragments in lagging strand DNA synthesis. Direct physical interaction between this protein and AP endonuclease 1 during long-patch base excision repair provides coordinated loading of the proteins onto the substrate, thus passing the substrate from one enzyme to another. The protein is a member of the XPG/RAD2 endonuclease family and is one of ten proteins essential for cell-free DNA replication. DNA secondary structure can inhibit flap processing at certain trinucleotide repeats in a length-dependent manner by concealing the 5' end of the flap that is necessary for both binding and cleavage by the protein encoded by this gene. Therefore, secondary structure can deter the protective function of this protein, leading to site-specific trinucleotide expansions. |
| Source : |
HEK293 |
| Species : |
Human |
| Tag : |
Myc/DDK |
| Molecular Mass : |
42.6 kDa |
| AA Sequence : |
MGIQGLAKLIADVAPSAIRENDIKS YFGRKVAIDASMSIYQFLIAVRQGG DVLQNEEGETTSHLMGMFYRTIRMM ENGIKPVYVFDGKPPQLKSGELAKR SERRAEAEKQLQQAQAAGAEQEVEK FTKRLVKVTKQHNDECKHLLSLMGI PYLDAPSEAEASCAALVKAGKVYAA ATEDMDCLTFGSPVLMRHLTASEAK KLPIQEFHLSRILQELGLNQEQFVD LCILLGSDYCESIRGIGPKRAVDLI QKHKSIEEIVRRLDPNKYPVPENWL HKEAHQLFLEPEVLDPESVELKWSE PNEEELIKFMCGEKQFSEERIRSGV KRLSKSRQGSTQGRLDDFFKVTGSL SSAKRKEPEPKGSTKKKAKTGAAGK FKRGKTRTRPLEQKLISEEDLAAND ILDYKDDDDKV |
| Purity : |
> 80% as determined by SDS-PAGE and Coomassie blue staining |
| Stability : |
Stable for 3 months from receipt of products under proper storage and handling conditions. |
| Storage : |
Store at -80 centigrade. Avoid repeated freeze-thaw cycles. |
| Concentration : |
50 μg/mL as determined by BCA |
| Storage Buffer : |
100 mM glycine, 25 mM Tris-HCl, pH 7.3. |
