Recombinant Human FGF2 Protein

• Cat.No.: FGF2-122H
• Product Overview: Recombinant Human FGF2 (Fibroblast Growth Factor 2) is produced by E. coli. It is a single, nonglycosylated, polypeptide chain containing 155 amino acids. The sequence of the first six N-terminal amino acids was determined to be MPALPE.

Specification

• Description: FGF-basic is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein functions as a modifier of endothelial cell migration and proliferation, as well as an angiogenic factor. It acts as a mitogen for a variety of mesoderm- and neuroectoderm-derived cells in vitro, thus is thought to be involved in organogenesis. Three alternatively spliced variants encoding different isoforms have been described. The heparin-binding growth factors are angiogenic agents in vivo and are potent mitogens for a variety of cell types in vitro. There are differences in the tissue distribution and concentration of these 2 growth factors. Fibroblast Growth Factor-2 Human Recombinant (FGF-2) produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 155 amino acids and having a molecular mass of 17250 Dalton. Human FGF2 is purified by proprietary chromatographic techniques.
• Source: E. coli
• Species: Human
• Form: The protein was lyophilized from a concentrated (0.5 mg/ml) solution of PBS.
• Molecular Mass: Predicted Molecular Weight: 17.25 Kda
• AA Sequence: Gly132-Ser288
• Endotoxin: Less than 0.1 ng/ug (1 EU/ug).
• Purity: >98.0% as determined by Gel filtration.
• Storage: Lyophilized protein should be stored at < -20 centigrade, though stable at room temperature for 3 weeks.
  Aliquots of reconstituted samples should be stored < -20 centigrade for 3 months.
• Reconstitution: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
  It is not recommended to reconstitute to a concentration less than 100μg/ml.
  Dissolve the lyophilized protein in distilled water.
  Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Quality Statement: Purity: >98.0% as determined by SEC-HPLC under non denaturing conditions using 25 nM Tris-HCl+150 nM NaCl, pH 8.0, and by reducing and non-reducing SDS-PAGE gel. Less than 10% dimers as determined by SEC-HPLC and no aggregates.
• Shipping: The product is shipped at ambient temperature.

Gene Information

• Gene Name: FGF2 fibroblast growth factor 2 [ Homo sapiens (human) ]
• Official Symbol: FGF2
• Synonyms: FGF-2; bFGF; FGFB; HBGF-2; BFGF; Fibroblast growth factor 2; Basic fibroblast growth factor; Heparin-binding growth factor 2
• Gene ID: 2247
• mRNA Refseq: NM_002006.6
• Protein Refseq: NP_001997.5
• MIM: 134920
• UniProt ID: P09038