Recombinant Human FST protein(Met1-Trp344), hFc-tagged

• Cat.No.: FST-3848H
• Product Overview: Recombinant Human FST (NP_037541.1) (Met 1-Trp 344) was expressed in HEK293, fused with the Fc region of Human IgG1 at the C-terminus.

Specification

• Source: HEK293
• Species: Human
• Tag: C-hFc
• Protein length: Met1-Trp344
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: 1. Measured by its ability to bind human INHBA-his in a functional ELISA. 2. Measured by its ability to bind mouse INHBA-his in a functional ELISA. 3. Measured by its ability to neutralize Activin-mediated inhibition on MPC11 cell proliferation. The ED50 for this effect is typically 0.5-3 μg/mL in the presence of 10 ng/ml Recombinant Human ctivin A.
• Molecular Mass: The secreted recombinant human FST/Fc is a disulfide-linked homodimer. The reduced monomer consists of 556 amino acids and has a predicted molecular mass of 61.7 kDa. As a result of glycosylation, rh FST/Fc monomer migrates as an approximately 70 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 85 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: FST follistatin [ Homo sapiens ]
• Official Symbol: FST
• Synonyms: FST; follistatin; FS; activin-binding protein; follistatin isoform FST317
• Gene ID: 10468
• mRNA Refseq: NM_006350
• Protein Refseq: NP_006341
• MIM: 136470
• UniProt ID: P19883

Reviews

11/09/2022

The proteins accepted were of very high purity and were put into experiments with satisfactory results.

02/26/2022

Very professional customer service, fully understanding the needs of the customer and giving professional advice.

03/14/2021

The FST proteins purchased from your company is suitable for WB.

Q&As

What are the effects of overexpressing FST?

Overexpression of FST promotes muscle growth.

What is the function of FST?

FST exists as a monomer and plays a role in folliculogenesis and luteinization.

What is the relationship between FST and Myostatin?

FST binds to Myostatin and inhibits its activity.

In which tissues and organs is FST expressed?

FST is widely expressed in ovary, skin and skeletal muscle.