Recombinant Human FZR1 Protein (1-493 aa), His-SUMO-tagged

• Cat.No.: FZR1-1160H
• Product Overview: Recombinant Human FZR1 Protein (1-493 aa) is produced by E. coli expression system. This protein is fused with a 6xHis-SUMO tag at the N-terminal. Research Area: Cell Biology. Protein Description: Full Length of Isoform 2.

Specification

• Description: Key regulator of ligase activity of the anaphase promoting complex/cyclosome (APC/C), which confers substrate specificity upon the complex. Associates with the APC/C in late mitosis, in replacent of CDC20, and activates the APC/C during anaphase and telophase. The APC/C rains active in degrading substrates to ensure that positive regulators of the cell cycle do not accumulate praturely. At the G1/S transition FZR1 is phosphorylated, leading to its dissociation from the APC/C. Following DNA damage, it is required for the G2 DNA damage checkpoint: its dephosphorylation and reassociation with the APC/C leads to the ubiquitination of PLK1, preventing entry into mitosis.
• Source: E. coli
• Species: Human
• Tag: His-SUMO
• Form: Tris-based buffer,50% glycerol
• Molecular Mass: 70.8 kDa
• Protein length: 1-493 aa
• AA Sequence: MDQDYERRLLRQIVIQNENTMPRVT EMRRTLTPASSPVSSPSKHGDRFIP SRAGANWSVNFHRINENEKSPSQNR KAKDATSDNGKDGLAYSALLKNELL GAGIEKVQDPQTEDRRLQPSTPEKK GLFTYSLSTKRSSPDDGNDVSPYSL SPVSNKSQKLLRSPRKPTRKISKIP FKVLDAPELQDDFYLNLVDWSSLNV LSVGLGTCVYLWSACTSQVTRLCDL SVEGDSVTSVGWSERGNLVAVGTHK GFVQIWDAAAGKKLSMLEGHTARVG ALAWNAEQLSSGSRDRMILQRDIRT PPLQSERRLQGHRQEVCGLKWSTDH QLLASGGNDNKLLVWNHSSLSPVQQ YTEHLAAVKAIAWSPHQHGLLASGG GTADRCIRFWNTLTGQPLQCIDTGS QVCNLAWSKHANELVSTHGYSQNQI LVWKYPSLTQVAKLTGHSYRVLYLA MSPDGEAIVTGAGDETLRFWNVFSK TRSTKESVSVLNLFTRIR
• Purity: > 90% as determined by SDS-PAGE.
• Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 centigrade for up to one week.
• Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 centigrade/-80 centigrade. The shelf life of lyophilized form is 12 months at -20 centigrade/-80 centigrade.
• Concentration: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Gene Information

• Gene Name: FZR1 fizzy/cell division cycle 20 related 1 (Drosophila) [ Homo sapiens ]
• Official Symbol: FZR1
• Synonyms: FZR1; CDC20C; CDH1; FZR; FZR2; HCDH; HCDH1; KIAA1242; CDC20-like 1b
• Gene ID: 51343
• mRNA Refseq: NM_001136197
• Protein Refseq: NP_001129669
• MIM: 603619
• UniProt ID: Q9UM11