Recombinant Human GAPDH, GST-tagged

• Cat.No.: GAPDH-117H
• Product Overview: Human GAPDH full-length ORF (1 a.a. - 335 a.a.) recombinant protein with GST-tag at N-terminal.

Specification

• Description: The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains. Many pseudogenes similar to this locus are present in the human genome. Two transcript variants encoding different isoforms have been found for this gene.
• Source: Wheat germ
• Species: Human
• Tag: GST
• Molecular Mass: 62.59 kDa
• AA Sequence: MGKVKVGVNGFGRIGRLVTRAAFNS GKVDIVAINDPFIDLNYMVYMFQYD STHGKFHGTVKAENGKLVINGNPIT IFQERDPSKIKWGDAGAEYVVESTG VFTTMEKAGAHLQGGAKRVIISAPS ADAPMFVMGVNHEKYDNSLKIISN ASCTTNCLAPLAKVIHDNFGIVEGL MTTVHAITATQKTVDGPSGKLWRDG R GALQNIIPASTGAAKAVGKVIPE LNGKLTGMAFRVPTANVSVVDLTCR LEKPAKYDDIKKVVKQASEGPLKGI LGYTEHQVVSSDFNSDTHSSTFDAG AG IALNDHFVKLISWYDNEFGYSNRVV DLMAHMASKE
• Applications: ELISA; WB-Re; AP; Array
• Storage: Store at -80°C. Aliquot to avoid repeated freezing and thawing.
• Storage Buffer: 50 mM Tris-HCI, 10 mM reduced Glutathione, pH=8.0 in the elution buffer.

Gene Information

• Gene Name: GAPDH glyceraldehyde-3-phosphate dehydrogenase [ Homo sapiens (human) ]
• Official Symbol: GAPDH
• Synonyms: GAPDH; G3PD; GAPD; glyceraldehyde-3-phosphate dehydrogenase; aging-associated gene 9 protein; peptidyl-cysteine S-nitrosylase GAPDH; EC 1.2.1.12
• Gene ID: 2597
• mRNA Refseq: NM_002046
• Protein Refseq: NP_002037
• MIM: 138400
• UniProt ID: P04406
• Chromosome Location: 12p13
• Pathway: Alzheimer''s disease; Biosynthesis of amino acids; Carbon metabolism
• Function: NAD binding; glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity; identical protein binding

Reviews

07/14/2022

Customer service was patient, prompt and accurate in answering my queries.

11/26/2021

High protein concentration, good quality, worth buying!

01/02/2021

The product brochure is very detailed and has comprehensive basic information about the GAPDH protein.

Q&As

Is GAPDH expression stable across tissues?

GAPDH is highly expressed in all tissues with relatively stable expression and is often used as an internal reference protein.

What diseases has GAPDH been closely associated with the study of?

GAPDH is closely related to the study of hepatocellular carcinoma and can be used as one of the indicators for the diagnosis of hepatocellular carcinoma.

What is the relationship between GAPDH and HDAC1?

GAPDH binds to HDAC1.

What are the biological functions of GAPDH?

GAPDH binds to matrix proteins, regulates host immune responses, and plays a role in pathogen virulence and surface localization, among others.

What are the effects of affecting protein expression of GAPDH?

Interference with GAPDH protein expression results in telomere shortening.