Recombinant Human GAPDH Protein(Met 1-Glu 335), His-tagged

• Cat.No.: GAPDH-014H
• Product Overview: Recombinant Human GAPDH Protein(P04406) (Met 1-Glu 335) was expressed in E. coli, with a polyhistidine tag at the N-terminus.

Specification

• Source: E. coli
• Species: Human
• Tag: N-His
• Protein length: Met 1-Glu 335
• Form: Supplied as sterile 50mM Tris, 30% glycerol, pH 7.5.
• Bio-activity: Measured by its ability to catalyze D-glyceraldehyde 3-phosphate to 3-phosphoglycerate, The specific activity is >2500pmols/min/ug.
• Molecular Mass: The recombinant human GAPDH comprises 346 amino acids and migrates as an approximately 38 kDa band in SDS-PAGE under reducing conditions as predicted.
• Purity: > 93 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: GAPDH glyceraldehyde-3-phosphate dehydrogenase [ Homo sapiens ]
• Official Symbol: GAPDH
• Synonyms: GAPDH; glyceraldehyde-3-phosphate dehydrogenase; GAPD; aging-associated gene 9 protein; peptidyl-cysteine S-nitrosylase GAPDH; G3PD; MGC88685
• Gene ID: 2597
• mRNA Refseq: NM_001256799
• Protein Refseq: NP_001243728
• MIM: 138400
• UniProt ID: P04406

Reviews

07/14/2022

Customer service was patient, prompt and accurate in answering my queries.

11/26/2021

High protein concentration, good quality, worth buying!

01/02/2021

The product brochure is very detailed and has comprehensive basic information about the GAPDH protein.

Q&As

Is GAPDH expression stable across tissues?

GAPDH is highly expressed in all tissues with relatively stable expression and is often used as an internal reference protein.

What diseases has GAPDH been closely associated with the study of?

GAPDH is closely related to the study of hepatocellular carcinoma and can be used as one of the indicators for the diagnosis of hepatocellular carcinoma.

What is the relationship between GAPDH and HDAC1?

GAPDH binds to HDAC1.

What are the biological functions of GAPDH?

GAPDH binds to matrix proteins, regulates host immune responses, and plays a role in pathogen virulence and surface localization, among others.

What are the effects of affecting protein expression of GAPDH?

Interference with GAPDH protein expression results in telomere shortening.