Recombinant Human GAPDH protein, T7/His-tagged

• Cat.No.: GAPDH-231H
• Product Overview: Recombinant human GAPDH cDNA ( 2 – 335 aa, Isoform-I ) fused with T7-His-TEV cleavage site Tag at N-terminal was expressed in E. coli.

Specification

• Source: E. coli
• Species: Human
• Tag: T7/His
• Form: 0.2 mg/ml, sterile-filtered, in 20 mM pH 8.0 Tris-HCl Buffer, with proprietary formulation of NaCl, KCl, EDTA, arginine, DTT and Sucrose.
• AA Sequence: MASMTGGQQMGRGHHHHHHENLYFQ GGEFGKVKVGVNGFGRIGRLVTRAA FNSGKVDIVAINDPFIDLNYMVYMF QYDSTHGKFHGTVKAENGKLVINGN PITIFQERDPSKIKWGDAGAEYVVE STGVFTTMEKAGAHLQGGAKRVIIS APSADAPMFVMGVNHEKYDNSLKII SNASCTTNCLAPLAKVIHDNFGIVE GLMTTVHAITATQKTVDGPSGKLWR DGRGALQNIIPASTGAAKAVGKVIP ELNGKLTGMAFRVPTANVSVVDLTC RLEKPAKYDDIKKVVKQASEGPLKG ILGYTEHQVVSSDFNSDTHSSTFDA GAGIALNDHFVKLISWYDNEFGYSN RVVDLMAHMASKE
• Purity: >90% by SDS-PAGE
• Storage: Keep at -80 centigrade for long term storage. Product is stable at 4 centigrade for at least 7 days.

Gene Information

• Gene Name: GAPDH glyceraldehyde-3-phosphate dehydrogenase [ Homo sapiens ]
• Official Symbol: GAPDH
• Synonyms: GAPDH; glyceraldehyde-3-phosphate dehydrogenase; GAPD; aging-associated gene 9 protein; peptidyl-cysteine S-nitrosylase GAPDH; G3PD; MGC88685
• Gene ID: 2597
• mRNA Refseq: NM_001256799
• Protein Refseq: NP_001243728
• MIM: 138400
• UniProt ID: P04406
• Chromosome Location: 12p13.31
• Pathway: Alzheimers disease, organism-specific biosystem; Alzheimers disease, conserved biosystem; Androgen Receptor Signaling Pathway, organism-specific biosystem; Gluconeogenesis, organism-specific biosystem; Gluconeogenesis, oxaloacetate =>fructose-6P, organism-specific biosystem; Gluconeogenesis, oxaloacetate =>
• Function: NAD binding; NADP binding; glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity; glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity; oxidoreductase activity; peptidyl-cysteine S-nitrosylase activity; protein b

Reviews

07/14/2022

Customer service was patient, prompt and accurate in answering my queries.

11/26/2021

High protein concentration, good quality, worth buying!

01/02/2021

The product brochure is very detailed and has comprehensive basic information about the GAPDH protein.

Q&As

Is GAPDH expression stable across tissues?

GAPDH is highly expressed in all tissues with relatively stable expression and is often used as an internal reference protein.

What diseases has GAPDH been closely associated with the study of?

GAPDH is closely related to the study of hepatocellular carcinoma and can be used as one of the indicators for the diagnosis of hepatocellular carcinoma.

What is the relationship between GAPDH and HDAC1?

GAPDH binds to HDAC1.

What are the biological functions of GAPDH?

GAPDH binds to matrix proteins, regulates host immune responses, and plays a role in pathogen virulence and surface localization, among others.

What are the effects of affecting protein expression of GAPDH?

Interference with GAPDH protein expression results in telomere shortening.