Recombinant Human GCA, His-tagged

• Cat.No.: GCA-26242TH
• Product Overview: Recombinant full length Human Grancalcin with an N terminal His tag; 237 amino acids with tag, Predicted MWt 26.1 kDa.

Specification

• Description: This gene product, grancalcin, is a calcium-binding protein abundant in neutrophils and macrophages. It belongs to the penta-EF-hand subfamily of proteins which includes sorcin, calpain, and ALG-2. Grancalcin localization is dependent upon calcium and magnesium. In the absence of divalent cation, grancalcin localizes to the cytosolic fraction; with magnesium alone, it partitions with the granule fraction; and in the presence of magnesium and calcium, it associates with both the granule and membrane fractions, suggesting a role for grancalcin in granule-membrane fusion and degranulation.
• Protein length: 217 amino acids
• Conjugation: HIS
• Molecular Weight: 26.100kDa inclusive of tags
• Source: E. coli
• Form: Liquid
• Purity: >95% by SDS-PAGE
• Storage buffer: Preservative: NoneConstituents: 20% Glycerol, 0.1M Sodium chloride, 20mM Tris HCl, pH 8.0
• Storage: Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Sequences of amino acids: MGSSHHHHHHSSGLVPRGSHMAYPG YGGGFGNFSIQVPGMQMGQPVPETG PAILLDGYSGPAYSDTYSSAGDSVY TYFSAVAGQDGEVDAEELQRCLTQS GINGTYSPFSLETCRIMIAMLDRDH TGKMGFNAFKELWAALNAWKENFMT VDQDGSGTVEHHELRQAIGLMGYRL SPQTLTTIVKRYSKNGRIFFDDYVA CCVKLRALTDFFRKRDHLQQGSANF IYDDFLQGTMAI

Gene Information

• Gene Name: GCA grancalcin, EF-hand calcium binding protein [ Homo sapiens ]
• Official Symbol: GCA
• Synonyms: GCA; grancalcin, EF-hand calcium binding protein; grancalcin, EF hand calcium binding protein; grancalcin; GCL
• Gene ID: 25801
• mRNA Refseq: NM_012198
• Protein Refseq: NP_036330
• MIM: 607030
• Uniprot ID: P28676
• Chromosome Location: 2p14-q14.3
• Function: calcium ion binding; protein homodimerization activity