Recombinant Human GCAT Protein, His-tagged

• Cat.No.: GCAT-2195H
• Product Overview: Recombinant Human GCAT Protein (Tyr224-Cys404) with a N-His tag was expressed in E. coli.

Specification

• Description: The degradation of L-threonine to glycine consists of a two-step biochemical pathway involving the enzymes L-threonine dehydrogenase and 2-amino-3-ketobutyrate coenzyme A ligase. L-Threonine is first converted into 2-amino-3-ketobutyrate by L-threonine dehydrogenase. This gene encodes the second enzyme in this pathway, which then catalyzes the reaction between 2-amino-3-ketobutyrate and coenzyme A to form glycine and acetyl-CoA. The encoded enzyme is considered a class II pyridoxal-phosphate-dependent aminotransferase. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 14.
• Source: E. coli
• Species: Human
• Tag: N-His
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 22.8 kDa
  Accurate Molecular Mass: 23 kDa
• Protein length: Tyr224-Cys404
• Endotoxin: <1.0 EU per 1µg (determined by the LAL method).
• Purity: > 95%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Storage Buffer: PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in PBS or others.

Gene Information

• Gene Name: GCAT glycine C-acetyltransferase [ Homo sapiens (human) ]
• Official Symbol: GCAT
• Synonyms: GCAT; glycine C-acetyltransferase; glycine C acetyltransferase (2 amino 3 ketobutyrate coenzyme A ligase); 2-amino-3-ketobutyrate coenzyme A ligase, mitochondrial; 2 amino 3 ketobutyrate coenzyme A ligase; KBL; AKB ligase; aminoacetone synthase; glycine acetyltransferase; 2-amino-3-ketobutyrate-CoA ligase; MGC23053
• Gene ID: 23464
• mRNA Refseq: NM_001171690
• Protein Refseq: NP_001165161
• MIM: 607422
• UniProt ID: O75600