Active Recombinant Human GIF Protein

• Cat.No.: GIF-256H
• Product Overview: Recombinant Human GIF was expressed in Arabidopsis thaliana.

Specification

• Description: This gene is a member of the cobalamin transport protein family. It encodes a glycoprotein secreted by parietal cells of the gastric mucosa and is required for adequate absorption of vitamin B12. Vitamin B12 is necessary for erythrocyte maturation and mutations in this gene may lead to congenital pernicious anemia.
• Source: Arabidopsis thaliana
• Species: Human
• Form: Dialysed against HPLC-grade water and sterile filtered, lyophilized apoIF (1.2mg), > 90% solids.
• Bio-activity: Binds 1 mole B12 per 1 mole rhIF, measured by a specific cobalamin-binding assay in HPLC-SEC
• Protein length: 399 amino acids
• AA Sequence: Has a predicted molar mass of 50 kDa.
• Purity: > 95% as determined by HPLC-SEC and SDS-PAGE
• Notes: The intrinsic factor is purified as the full-length protein using chromatographic techniques, unoccupied, as apoIF50 (free of B12 or other bound molecules).
  The protein does not contain any N- or C-terminal tags often used to facilitate purification, av
• Storage: Lyophilized sample stable at room temperature.
  Freeze reconstituted samples at -20 centigrade and avoid repeated freeze-thaw cycles.
• Reconstitution: Reconstitute in water or recommended buffer, e.g. 0.15M phosphate (pH7.5), depending upon your application.

Gene Information

• Gene Name: GIF gastric intrinsic factor (vitamin B synthesis) [ Homo sapiens ]
• Official Symbol: GIF
• Synonyms: GIF; gastric intrinsic factor (vitamin B synthesis); gastric intrinsic factor; IF; IFMH; INF; TCN3; intrinsic factor
• Gene ID: 2694
• mRNA Refseq: NM_005142
• Protein Refseq: NP_005133
• MIM: 609342
• UniProt ID: P27352