| Description : |
This gene encodes a member of the GIT protein family, which interact with G protein-coupled receptor kinases and possess ADP-ribosylation factor (ARF) GTPase-activating protein (GAP) activity. GIT proteins traffic between cytoplasmic complexes, focal adhesions, and the cell periphery, and interact with Pak interacting exchange factor beta (PIX) to form large oligomeric complexes that transiently recruit other proteins. GIT proteins regulate cytoskeletal dynamics and participate in receptor internalization and membrane trafficking. This gene has been shown to repress lamellipodial extension and focal adhesion turnover, and is thought to regulate cell motility. This gene undergoes extensive alternative splicing to generate multiple isoforms, but the full-length nature of some of these variants has not been determined. The various isoforms have functional differences, with respect to ARF GAP activity and to G protein-coupled receptor kinase 2 binding. |
| Source : |
HEK293 |
| Species : |
Human |
| Tag : |
Myc/DDK |
| Molecular Mass : |
52.6 kDa |
| AA Sequence : |
MSKRLRSSEVCADCSGPDPSWASVN RGTFLCDECCSVHRSLGRHISQVRH LKHTPWPPTLLQMVETLYNNGANSI WEHSLLDPASIMSGRRKANPQDKVH PNKAEFIRAKYQMLAFVHRLPCRDD DSVTAKDLSKQLHSSVRTGNLETCL RLLSLGAQANFFHPEKGNTPLHVAS KAGQILQAELLAVYGADPGTQDSSG KTPVDYARQGGHHELAERLVEIQYE LTDRLAFYLCGRKPDHKNGQHFIIP QMADSSLDLSELAKAAKKKLQSLSN HLFEELAMDMYDEVDRRETDAVWLA TQNHSALVTETTVVPFLPVNPEYSS TRNQGRQKLARFNAHEFATLVIDIL SDAKRRQQGSSLSGSKDNVELILKT INNQHSVESQDNDQPDYDSVASDED TDLETTASKTNRQKSLDSDLSDGPV TVQEFMEVKNALVASEAKIQQLMKV NNNLSDELRIMQKKLLGKDANTRTR PLEQKLISEEDLAANDILDYKDDDD KV |
| Purity : |
> 80% as determined by SDS-PAGE and Coomassie blue staining |
| Stability : |
Stable for 3 months from receipt of products under proper storage and handling conditions. |
| Storage : |
Store at -80 centigrade. Avoid repeated freeze-thaw cycles. |
| Concentration : |
50 μg/mL as determined by BCA |
| Storage Buffer : |
100 mM glycine, 25 mM Tris-HCl, pH 7.3. |
