| Description : |
This gene encodes glutamate dehydrogenase, which is a mitochondrial matrix enzyme that catalyzes the oxidative deamination of glutamate to alpha-ketoglutarate and ammonia. This enzyme has an important role in regulating amino acid-induced insulin secretion. It is allosterically activated by ADP and inhibited by GTP and ATP. Activating mutations in this gene are a common cause of congenital hyperinsulinism. Alternative splicing of this gene results in multiple transcript variants. The related glutamate dehydrogenase 2 gene on the human X-chromosome originated from this gene via retrotransposition and encodes a soluble form of glutamate dehydrogenase. Related pseudogenes have been identified on chromosomes 10, 18 and X. |
| Source : |
HEK293 |
| Species : |
Human |
| Tag : |
Myc/DDK |
| Molecular Mass : |
61.4 kDa |
| AA Sequence : |
MYRYLGEALLLSRAGPAALGSASAD SAALLGWARGQPAAAPQPGLALAAR RHYSEAVADREDDPNFFKMVEGFFD RGASIVEDKLVEDLRTRESEEQKRN RVRGILRIIKPCNHVLSLSFPIRRD DGSWEVIEGYRAQHSQHRTPCKGGI RYSTDVSVDEVKALASLMTYKCAVV DVPFGGAKAGVKINPKNYTDNELEK ITRRFTMELAKKGFIGPGIDVPAPD MSTGEREMSWIADTYASTIGHYDIN AHACVTGKPISQGGIHGRISATGRG VFHGIENFINEASYMSILGMTPGFG DKTFVVQGFGNVGLHSMRYLHRFGA KCIAVGESDGSIWNPDGIDPKELED FKLQHGSILGFPKAKPYEGSILEAD CDILIPAASEKQLTKSNAPRVKAKI IAEGANGPTTPEADKIFLERNIMVI PDLYLNAGGVTVSYFEWLKNLNHVS YGRLTFKYERDSNYHLLMSVQESLE RKFGKHGGTIPIVPTAEFQDRISGA SEKDIVHSGLAYTMERSARQIMRTA MKYNLGLDLRTAAYVNAIEKVFKVY NEAGVTFTTRTRPLEQKLISEEDLA ANDILDYKDDDDKV |
| Purity : |
> 80% as determined by SDS-PAGE and Coomassie blue staining |
| Stability : |
Stable for 3 months from receipt of products under proper storage and handling conditions. |
| Storage : |
Store at -80 centigrade. Avoid repeated freeze-thaw cycles. |
| Concentration : |
50 μg/mL as determined by BCA |
| Storage Buffer : |
100 mM glycine, 25 mM Tris-HCl, pH 7.3. |
