Recombinant Human Glyceraldehyde-3-phosphate dehydrogenase

• Cat.No.: GAPDH-115H
• Product Overview: Recombinant GAPDH protein was expressed in E.coli and purified by using conventional chromatography techniques, 36kDa (335aa).

Specification

• Cat. No.: GAPDH-115H
• Description: Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a catalytic enzyme commonly known to be involved in glycolysis. The enzyme exists as a tetramer composed of 36-kDa subunits and has various intracellular functions. GAPDH catalyzes the reversible reduction of 1,3-bisphosphoglycerate to glyceraldehyde 3-phosphophate in the presence of NADPH. Besides functioning as a glycolytic enzyme in cytoplasm, evidence suggests that mammalian GAPDH is also involved in a great number of intracellular processes such as membrane fusion, microtubule bundling, phosphotransferase activity, nuclear RNA export, DNA replication and DNA repair.
• Sequences of amino acids: MGKVKVGVNG FGRIGRLVTR AAFNSGKVDI VAINDPFIDL NYMVYMFQYD STHGKFHGTV KAENGKLVIN GNPITIFQER DPSKIKWGDA GAEYVVESTG VFTTMEKAGA HLQGGAKRVI ISAPSADAPM FVMGVNHEKY DNSLKIISNA SCTTNCLAPL AKVIHDNFGI VEGLMTTVHA ITATQKTVDG PSGKLWRDGR GALQNIIPAS TGAAKAVGKV IPELNGKLTG MAFRVPTANV SVVDLTCRLE KPAKYDDIKK VVKQASEGPL KGILGYTEHQ VVSSDFNSDT HSSTFDAGAG IALNDHFVKL ISWYDNEFGY SNRVVDLMAH MASKE
• Formulation: Liquid. In 20 mM Tris-HCl buffer (pH 8.0) containing 1 mM DTT, 1 mM EDTA, 20% glycerol
• Purity: > 95% by SDS - PAGE
• Concentration: 1 mg/ml (determined by Bradford assay)
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• Publication:
  Myeloperoxidase catalyzes the conjugation of serotonin to thiols via free radicals and tryptamine-4, 5-dione (2012)

Gene Information

• Gene Name: GAPDH glyceraldehyde-3-phosphate dehydrogenase [ Homo sapiens ]
• Synonyms: glyceraldehyde-3-phosphate dehydrogenase; G3PD; GAPD; MGC88685; GAPDH;glyceraldehyde-3-phosphate dehydrogenase; OTTHUMP00000174431; EC 1.2.1.12; OTTHUMP00000174432; aging-associated gene 9 protein; glyceraldehyde 3-phosphate dehydrogenase
• Gene ID: 2597
• mRNA Refseq: NM_002046
• Protein Refseq: NP_002037
• MIM: 138400
• UniProt ID: P04406
• Chromosome Location: 12p13
• Pathway: Alzheimer"s disease; Dentatorubropallidoluysian atrophy (DRPLA); Glycolysis / Gluconeogenesis; Huntington"s disease; Neurodegenerative Disorders; Metabolism of carbohydrates
• Function: NAD or NADH binding; glyceraldehyde-3-phosphate dehydrogenase (phosphorylating) activity; oxidoreductase activity; protein binding

Reviews

07/14/2022

Customer service was patient, prompt and accurate in answering my queries.

11/26/2021

High protein concentration, good quality, worth buying!

01/02/2021

The product brochure is very detailed and has comprehensive basic information about the GAPDH protein.

Q&As

Is GAPDH expression stable across tissues?

GAPDH is highly expressed in all tissues with relatively stable expression and is often used as an internal reference protein.

What diseases has GAPDH been closely associated with the study of?

GAPDH is closely related to the study of hepatocellular carcinoma and can be used as one of the indicators for the diagnosis of hepatocellular carcinoma.

What is the relationship between GAPDH and HDAC1?

GAPDH binds to HDAC1.

What are the biological functions of GAPDH?

GAPDH binds to matrix proteins, regulates host immune responses, and plays a role in pathogen virulence and surface localization, among others.

What are the effects of affecting protein expression of GAPDH?

Interference with GAPDH protein expression results in telomere shortening.