Recombinant Human GPD1 Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: GPD1-2440H
• Product Overview: GPD1 MS Standard C13 and N15-labeled recombinant protein (NP_005267) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: This gene encodes a member of the NAD-dependent glycerol-3-phosphate dehydrogenase family. The encoded protein plays a critical role in carbohydrate and lipid metabolism by catalyzing the reversible conversion of dihydroxyacetone phosphate (DHAP) and reduced nicotine adenine dinucleotide (NADH) to glycerol-3-phosphate (G3P) and NAD+. The encoded cytosolic protein and mitochondrial glycerol-3-phosphate dehydrogenase also form a glycerol phosphate shuttle that facilitates the transfer of reducing equivalents from the cytosol to mitochondria. Mutations in this gene are a cause of transient infantile hypertriglyceridemia. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
• Source: HEK293
• Species: Human
• Tag: Myc/DDK
• Molecular Mass: 37.6 kDa
• AA Sequence: MASKKVCIVGSGNWGSAIAKIVGGN AAQLAQFDPRVTMWVFEEDIGGKKL TEIINTQHENVKYLPGHKLPPNVVA VPDVVQAAEDADILIFVVPHQFIGK ICDQLKGHLKANATGISLIKGVDEG PNGLKLISEVIGERLGIPMSVLMGA NIASEVADEKFCETTIGCKDPAQGQ LLKELMQTPNFRITVVQEVDTVEIC GALKNVVAVGAGFCDGLGFGDNTKA AVIRLGLMEMIAFAKLFCSGPVSSA TFLESCGVADLITTCYGGRNRKVAE AFARTGKSIEQLEKELLNGQKLQGP ETARELYSILQHKGLVDKFPLFMAV YKVCYEGQPVGEFIHCLQNHPEHMT RTRPLEQKLISEEDLAANDILDYKD DDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: GPD1 glycerol-3-phosphate dehydrogenase 1 [ Homo sapiens (human) ]
• Official Symbol: GPD1
• Synonyms: GPD1; glycerol-3-phosphate dehydrogenase 1 (soluble); glycerol-3-phosphate dehydrogenase [NAD(+)], cytoplasmic; glycerophosphate dehydrogenase; glycerol-3-phosphate dehydrogenase [NAD+], cytoplasmic; GPD-C; HTGTI; GPDH-C; FLJ26652
• Gene ID: 2819
• mRNA Refseq: NM_005276
• Protein Refseq: NP_005267
• MIM: 138420
• UniProt ID: P21695