Recombinant Human GPER Protein, His&GST-tagged

• Cat.No.: GPER-1580H
• Product Overview: Recombinant Human GPER Protein (Met1-Ser62) with N-His&GST tag was expressed in E. coli.

Specification

• Description: This gene encodes a multi-pass membrane protein that localizes to the endoplasmic reticulum and a member of the G-protein coupled receptor 1 family. This receptor binds estrogen and activates multiple downstream signaling pathways, leading to stimulation of adenylate cyclase and an increase in cyclic AMP levels, while also promoting intracellular calcium mobilization and synthesis of phosphatidylinositol 3,4,5-trisphosphate in the nucleus. This protein therefore plays a role in the rapid nongenomic signaling events widely observed following stimulation of cells and tissues with estrogen. This receptor has been shown to play a role in diverse biological processes, including bone and nervous system development, metabolism, cognition, male fertility and uterine function.
• Source: E. coli
• Species: Human
• Tag: N-His&GST
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 36.5 kDa
  Accurate Molecular Mass: 40 kDa
• Protein length: Met1-Ser62
• Purity: > 90%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Storage Buffer: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: GPER G protein-coupled estrogen receptor 1 [ Homo sapiens (human) ]
• Official Symbol: GPER
• Synonyms: GPER; G protein-coupled estrogen receptor 1; CMKRL2, G protein coupled receptor 30, GPR30; G-protein coupled estrogen receptor 1; CEPR; DRY12; FEG 1; GPCR Br; LERGU; LERGU2; LyGPR; mER; heptahelix receptor; chemokine receptor-like 2; IL8-related receptor DRY12; membrane estrogen receptor; G protein-coupled receptor 30; G-protein coupled receptor 30; chemoattractant receptor-like 2; leucine rich protein in GPR30 3UTR; lymphocyte-derived G-protein coupled receptor; constitutively expressed peptide-like receptor; flow-induced endothelial G-protein coupled receptor 1; FEG-1; GPR30; CMKRL2; GPCR-Br; MGC99678
• Gene ID: 2852
• mRNA Refseq: NM_001039966
• Protein Refseq: NP_001035055
• MIM: 601805
• UniProt ID: Q99527