Recombinant Human GRAP Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: GRAP-2969H
• Product Overview: GRAP MS Standard C13 and N15-labeled recombinant protein (NP_006604) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: This gene encodes a member of the GRB2/Sem5/Drk family and functions as a cytoplasmic signaling protein which contains an SH2 domain flanked by two SH3 domains. The SH2 domain interacts with ligand-activated receptors for stem cell factor and erythropoietin, and facilitates the formation of a stable complex with the BCR-ABL oncoprotein. This protein also associates with the Ras guanine nucleotide exchange factor SOS1 (son of sevenless homolog 1) through its N-terminal SH3 domain. In general, it couples signals from receptor and cytoplasmic tyrosine kinases to the Ras signaling pathway.
• Source: HEK293
• Species: Human
• Tag: Myc/DDK
• Molecular Mass: 25.3 kDa
• AA Sequence: MESVALYSFQATESDELAFNKGDTL KILNMEDDQNWYKAELRGVEGFIPK NYIRVKPHPWYSGRISRQLAEEILM KRNHLGAFLIRESESSPGEFSVSVN YGDQVQHFKVLREASGKYFLWEEKF NSLNELVDFYRTTTIAKKRQIFLRD EEPLLKSPGACFAQAQFDFSAQDPS QLSFRRGDIIEVLERPDPHWWRGRS CGRVGFFPRSYVQPVHLTRTRPLEQ KLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: GRAP GRB2-related adaptor protein [ Homo sapiens (human) ]
• Official Symbol: GRAP
• Synonyms: GRAP; GRB2-related adaptor protein; GRB2-related adapter protein; growth factor receptor-bound protein 2-related adaptor protein; MGC64880
• Gene ID: 10750
• mRNA Refseq: NM_006613
• Protein Refseq: NP_006604
• MIM: 604330
• UniProt ID: Q13588