Recombinant Human GZMK protein(Ile27~Asn264), His-tagged

• Cat.No.: GZMK-3067H
• Product Overview: Recombinant Human GZMK protein(Ile27~Asn264), fused with N-terminal His Tag, was expressed in E.coli.

Specification

• Source: E.coli
• Species: Human
• Tag: N-His
• Protein length: Ile27~Asn264
• Form: PBS, pH7.4, containing 0.01% SKL, 5%Trehalose.
• Bio-activity: Granzyme K is a member of the granzyme family of the serine proteases found specifically in the cytotoxic granules of cytotoxic T lymphocytes (CTL) and natural killer (NK) cells. Human granzyme K is synthesized as a precursor (264 residues) with a signal peptide (residues 1- 24), a propeptide (residues 25-26) and a mature chain (residues 27-264). The purified recombinant human Granzyme K consists of residues 27 to 264 which activity was measured by its ability to cleaves a thioester substrate Z-Lys-SBzl • HCl. The reaction was performed in 0.05 M Tris, 0.15 M NaCl, 0.01% Triton X-100, pH 8.0 (assay buffer), initiated by addition 50 μL of various concentrations of GZMK (diluted by assay buffer) to 50 μL of 1.2 mM substrate and DTNB mixture. The final well serves as a negative control with no
  GZMK, replace with 50 μL assay buffer. Incubated at 25°C for 5min, then read at a wavelength of 405 nm. The specific activity of recombinant human Granzyme K is >220 pmol/min/μg.
• Molecular Mass: 27.1kDa
• Endotoxin: <1.0EU per 1μg (determined by the LAL method).
• Purity: > 90%
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Reconstitution: Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: GZMK granzyme K (granzyme 3; tryptase II) [ Homo sapiens ]
• Official Symbol: GZMK
• Synonyms: GZMK; granzyme K (granzyme 3; tryptase II); granzyme K (serine protease, granzyme 3; tryptase II); granzyme K; PRSS; TRYP2; NK-Tryp-2; granzyme 3; granzyme-3; tryptase II; fragmentin-3; NK-tryptase-2; granzyme K (serine protease, granzyme 3; tryptase II)
• Gene ID: 3003
• mRNA Refseq: NM_002104
• Protein Refseq: NP_002095
• MIM: 600784
• UniProt ID: P49863