Recombinant Human H6PD 293 Cell Lysate

• Cat.No.: H6PD-5651HCL

Specification

• Description: Antigen standard for hexose-6-phosphate dehydrogenase (glucose 1-dehydrogenase) (H6PD) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: H6PD hexose-6-phosphate dehydrogenase (glucose 1-dehydrogenase) [ Homo sapiens ]
• Official Symbol: H6PD
• Synonyms: H6PD; hexose-6-phosphate dehydrogenase (glucose 1-dehydrogenase); GDH, glucose dehyrogenase; GDH/6PGL endoplasmic bifunctional protein; G6PD, H form; glucose dehyrogenase; glucose dehydrogenase; glucose 1- dehydrogenase; 6-phosphogluconolactonase; glucose-6-phosphate dehydrogenase, salivary; GDH; G6PDH; MGC87643; DKFZp686A01246
• Gene ID: 9563
• mRNA Refseq: NM_004285
• Protein Refseq: NP_004276
• MIM: 138090
• UniProt ID: O95479
• Chromosome Location: 1p36
• Pathway: Metabolic pathways, organism-specific biosystem; Pentose phosphate pathway, organism-specific biosystem; Pentose phosphate pathway, conserved biosystem; Pentose phosphate pathway (Pentose phosphate cycle), organism-specific biosystem; Pentose phosphate pathway (Pentose phosphate cycle), conserved biosystem; Pentose phosphate pathway, oxidative phase, glucose 6P =>ribulose 5P, organism-specific biosystem
• Function: 6-phosphogluconolactonase activity; NADP binding; glucose 1-dehydrogenase [NAD(P)] activity; glucose-6-phosphate dehydrogenase activity; hydrolase activity; oxidoreductase activity; sugar binding