Recombinant Human HA, Fc-Tagged

• Cat.No.: HA-16H
• Product Overview: Recombinant Human HA, Fc-Tagged expressed in human cells comprises 754 amino acids with the predicted molecular mass of 85.5 kDa. As a result of glycosylation, it migrates as an approximately 110 kDa band in SDS-PAGE under reducing conditions.

Specification

• Cat. No.: HA -16H
• Description: Hemagglutinin (HA) is a Single-pass type I integral membrane glycoprotein from the influenza virus, and comprises over 80% of the envelope proteins present in the virus particle. It is a homotrimer of disulfide-linked HA1-HA2. Binding of HA to sialic acid-containing receptors on the surface of its target cell brings about the attachment of the virus particle to the cell and forms a endosome. Low pH in endosomes induce an irreversible conformational change in HA2, releasing the hydrophobic portion “fusion peptide”. After which, virus penetrates the cell and pours its contents including the RNA genome into the cytoplasm mediated by fusion of the endocytosed virus particle’s own membrane and the endosomal membrane. Hemagglutinin plays a major role in the determination of host range restriction and virulence. A DNA sequence encoding the extracellular domain of Influenza A virus (A/Anhui/1/2005(H5N1) (ABD28180.1) hemagglutinin (Met 1-Gln 527) with cleavage site mutated (HA1+HA2, uncleaved) was was fused with the C-terminal polyhistidine-tagged Fc region of mouse IgG1 at the C-terminus.
• Predicted N terminal: Asp 17
• Purity: > 97%, as determined by SDS-PAGE
• Formulation: Supplied as a 0.2μm filtered solution of 100mM Glycine,10mMNaCl,50mMTris,pH7.5
• Endotoxin: < 1.0 EU per 1μg cytokine as determined by the LAL method
• Reconstitution: Follow the instructions on the vial. Centrifuge the vial at 4℃ before opening to recover the entire contents.
• Stability: Samples are stable for up to twelve months from date of receipt -70°C
• Storage: Store it under sterile conditions at -70°C. It is recommended that the protein be aliquoted and be used as soon as possible.Avoid repeated freeze-thaw cycles.

Gene Information

• Gene Name: HA hemagglutinin [ Influenza A virus (A/Goose/Guangdong/1/96(H5N1)) ]
• Synonyms: HA; hemagglutinin; HA1; HA2
• Gene ID: 3654620
• Protein Refseq: YP_308669

Reviews

06/01/2021

The transport process does not affect the protein quality, and the stability is good.

11/27/2019

Higher solubility makes it easy to prepare the desired concentration.

Q&As

How about the structural characteristics of HA protein?

HA protein is a glycoprotein that contains thousands of carbohydrates and an amino acid chain consisting of 566 amino acid residues. The structure of HA protein has triple symmetry, consisting of three monomers that form a spiral.

Will genetic variants in HA proteins affect vaccine development?

Genetic variants in the HA protein may affect the development and production of influenza vaccines. Because HA protein is an important component of influenza viruses, different subtypes or variants of HA protein may require different vaccines to prepare.

Why is hemagglutinin protein related to viral replication?

This protein is one of the key factors in the replication process of influenza viruses. When a virus infects a host cell, the receptor-binding region of the HA protein binds to the receptor on the cell surface, allowing the virus to invade the host cell and replicate.

What are the subtypes of HA protein?

There are currently 18 known HA subtypes of influenza viruses, H1-H18. Among them, the H1, H2, H3, H5, H7, and H9 subtypes are associated with human viruses, while other subtypes are generally associated with animal viruses.

What are the physiological functions of HA protein?

The main physiological function of HA protein is to bind to host cells and mediate the entry of influenza viruses into host cells. It contains specific regions that bind glycoprotein receptors that allow the influenza virus to adhere to them. This is an important link that allows the virus to stably bind and enter the cell.

What laboratory techniques are HA proteins used in?

HA protein is widely used in viral research and drug development. Researchers can use HA proteins to test drugs such as influenza virus activators, antibodies and vaccines, and HA proteins are also widely used to develop novel immunoassays and treatments.