Recombinant Human HAAO Protein, His-tagged

• Cat.No.: HAAO-18H
• Product Overview: Recombinant Human HAAO Protein, fused to His-tag, was expressed in E. coli.

Specification

• Description: 3-Hydroxyanthranilate 3,4-dioxygenase is a monomeric cytosolic protein belonging to the family of intramolecular dioxygenases containing nonheme ferrous iron. It is widely distributed in peripheral organs, such as liver and kidney, and is also present in low amounts in the central nervous system. HAAO catalyzes the synthesis of quinolinic acid (QUIN) from 3-hydroxyanthranilic acid. QUIN is an excitotoxin whose toxicity is mediated by its ability to activate glutamate N-methyl-D-aspartate receptors. Increased cerebral levels of QUIN may participate in the pathogenesis of neurologic and inflammatory disorders. HAAO has been suggested to play a role in disorders associated with altered tissue levels of QUIN.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Liquid. In 20 mM Tris-HCl buffer (pH8.0) containing 0.15 M NaCl, 10% glycerol, 1mM DTT.
• Molecular Mass: ~35k Da
• AA Sequence: MGSSHHHHHHSSGLVPRGSHMGSHM ERRLGVRAWVKENRGSFQPPVCNKL MHQEQLKVMFIGGPNTRKDYHIEEG EEVFYQLEGDMVLRVLEQGKHRDVV IRQGEIFLLPARVPHSPQRFANTVG LVVERRRLETELDGLRYYVGDTMDV LFEKWFYCKDLGTQLAPIIQEFFSS EQYRTGKPIPDQLLKEPPFPLSTRS IMEPMSLDAWLDSHHRELQAGTPLS LFGDTYETQVIAYGQGSSEGLRQNV DVWLWQLEGSSVVTMGGRRLSLAPD DSLLVLAGTSYAWERTQGSVALSVT QDPACKKPLG
• Endotoxin: Less than 1 EU/mg as determined by LAL method.
• Purity: >95%
• Storage: Short Term Storage at 4 centigrade, Long Term, please prepare aliquots with 20% glycerol and store at -20 to -80 centigrade. Avoid freeze/thaw cycles.
• Concentration: 0.5 mg/ml
• Official Full Name: 3-hydroxyanthranilate 3,4-dioxygenase

Gene Information

• Gene Name: HAAO 3-hydroxyanthranilate 3,4-dioxygenase [ Homo sapiens (human) ]
• Official Symbol: HAAO
• Synonyms: HAO; 3-HAO; VCRL1; h3HAO
• Gene ID: 23498
• mRNA Refseq: NM_012205
• Protein Refseq: NP_036337
• MIM: 604521
• UniProt ID: P46952