Recombinant Human HAO1 Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: HAO1-3820H
• Product Overview: HAO1 MS Standard C13 and N15-labeled recombinant protein (NP_060015) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: This gene is one of three related genes that have 2-hydroxyacid oxidase activity yet differ in encoded protein amino acid sequence, tissue expression and substrate preference. Subcellular location of the encoded protein is the peroxisome. Specifically, this gene is expressed primarily in liver and pancreas and the encoded protein is most active on glycolate, a two-carbon substrate. The protein is also active on 2-hydroxy fatty acids. The transcript detected at high levels in pancreas may represent an alternatively spliced form or the use of a multiple near-consensus upstream polyadenylation site.
• Source: HEK293
• Species: Human
• Tag: Myc/DDK
• Molecular Mass: 40.9 kDa
• AA Sequence: MLPRLICINDYEQHAKSVLPKSIYD YYRSGANDEETLADNIAAFSRWKLY PRMLRNVAETDLSTSVLGQRVSMPI CVGATAMQRMAHVDGELATVRACQS LGTGMMLSSWATSSIEEVAEAGPEA LRWLQLYIYKDREVTKKLVRQAEKM GYKAIFVTVDTPYLGNRLDDVRNRF KLPPQLRMKNFETSTLSFSPEENFG DDSGLAAYVAKAIDPSISWEDIKWL RRLTSLPIVAKGILRGDDAREAVKH GLNGILVSNHGARQLDGVPATIDVL PEIVEAVEGKVEVFLDGGVRKGTDV LKALALGAKAVFVGRPIVWGLAFQG EKGVQDVLEILKEEFRLAMALSGCQ NVKVIDKTLVRKNPLAVSKITRTRP LEQKLISEEDLAANDILDYKDDDDK V
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: HAO1 hydroxyacid oxidase 1 [ Homo sapiens (human) ]
• Official Symbol: HAO1
• Synonyms: HAO1; hydroxyacid oxidase (glycolate oxidase) 1; GOX1; hydroxyacid oxidase 1; GOX; glycolate oxidase; (S)-2-hydroxy-acid oxidase; HAOX1; MGC142225; MGC142227
• Gene ID: 54363
• mRNA Refseq: NM_017545
• Protein Refseq: NP_060015
• MIM: 605023
• UniProt ID: Q9UJM8

Reviews

05/09/2022

Worked well in experiments.

11/28/2020

Great for academic research.

01/28/2020

Very satisfied, will buy again.

Q&As

How is HAO1 implicated in metabolic disorders?

Dysregulation of HAO1 is linked to metabolic disorders and fatty liver disease.

Are there existing or potential therapeutic strategies targeting HAO1?

Therapeutic approaches may include modulation of HAO1 activity or expression.

How does HAO1 contribute to fatty acid metabolism?

It oxidizes specific fatty acids, contributing to their breakdown.

What are the key biological functions of HAO1?

HAO1 is involved in peroxisomal fatty acid oxidation, crucial for lipid metabolism.

What are the potential consequences of HAO1 overexpression?

HAO1 overexpression may lead to oxidative stress and liver dysfunction.

What role does HAO1 play in liver function?

HAO1 supports liver function by participating in lipid catabolism.

How is HAO1 activity regulated in cells?

Cellular regulation of HAO1 involves transcriptional and post-translational mechanisms.