Recombinant Human HARS protein, His-tagged

• Cat.No.: HARS-2229H
• Product Overview: Recombinant Human HARS protein(P12081)(2-504aa), fused to N-terminal His tag, was expressed in Insect Cell.

Specification

• Source: Insect Cell
• Species: Human
• Tag: His
• Form: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
  If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
• Molecular Mass: 58.7 kDa
• Protein length: 2-504aa
• AA Sequence: AERAALEELVKLQGERVRGLKQQKA SAELIEEEVAKLLKLKAQLGPDESK QKFVLKTPKGTRDYSPRQMAVREKV FDVIIRCFKRHGAEVIDTPVFELKE TLMGKYGEDSKLIYDLKDQGGELLS LRYDLTVPFARYLAMNKLTNIKRYH IAKVYRRDNPAMTRGRYREFYQCDF DIAGNFDPMIPDAECLKIMCEILSS LQIGDFLVKVNDRRILDGMFAICGV SDSKFRTICSSVDKLDKVSWEEVKN EMVGEKGLAPEVADRIGDYVQQHGG VSLVEQLLQDPKLSQNKQALEGLGD LKLLFEYLTLFGIDDKISFDLSLAR GLDYYTGVIYEAVLLQTPAQAGEEP LGVGSVAAGGRYDGLVGMFDPKGRK VPCVGLSIGVERIFSIVEQRLEALE EKIRTTETQVLVASAQKKLLEERLK LVSELWDAGIKAELLYKKNPKLLNQ LQYCEEAGIPLVAIIGEQELKDGVI KLRSVTSREEVDVRREDLVEEIKRR TGQ
• Purity: Greater than 90% as determined by SDS-PAGE.
• Storage: Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
• Reconstitution: Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Gene Information

• Gene Name: HARS histidyl-tRNA synthetase [ Homo sapiens ]
• Official Symbol: HARS
• Synonyms: HARS; histidyl-tRNA synthetase; histidine--tRNA ligase, cytoplasmic; histidine tRNA ligase 1; cytoplasmic; HisRS; histidine translase; HRS; USH3B; FLJ20491
• Gene ID: 3035
• mRNA Refseq: NM_001258040
• Protein Refseq: NP_001244969
• MIM: 142810
• UniProt ID: P12081