Recombinant Human HDAC3 protein(Met1-Ile428), His&GST-tagged

• Cat.No.: HDAC3-3908H
• Product Overview: Recombinant full length of Human HDAC3 (O15379-1) (Met 1-Ile 428) was expressed in Insect Cells, fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Specification

• Source: Insect Cells
• Species: Human
• Tag: N-His&GST
• Protein length: Met1-Ile428
• Form: Lyophilized from sterile 20mM Tris, 500mM NaCl, 4mM GSH, pH 7.4 Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Molecular Mass: The recombinant human HDAC3/GST chimera consists of 666 amino acids and has a calculated molecular mass of 76.7 kDa. It migrates as an approximately 66 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 90 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: HDAC3 histone deacetylase 3 [ Homo sapiens ]
• Official Symbol: HDAC3
• Synonyms: HDAC3; histone deacetylase 3; HD3; RPD3; RPD3 2; SMAP45; RPD3-2
• Gene ID: 8841
• mRNA Refseq: NM_003883
• Protein Refseq: NP_003874
• MIM: 605166
• UniProt ID: O15379