| Description : |
E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates.. |
| Source : |
HEK293 |
| Species : |
Human |
| Tag : |
Myc/DDK |
| Molecular Mass : |
88.1 kDa |
| AA Sequence : |
MSEAVRVPSPATPLVVAAAAPEERK GKESEREKLPPIVSAGAGATAGLDR GAKGQISTFSSFISAVSPKKEAAEN RSSPAHLVFLNIKNVREPPPICLDV RQKQRTSMDASSSEMKAPVLPEPIL PIQPKTVKDFQEDVEKVKSSGDWKA VHDFYLTTFDSFPELNAAFKKDATA SFNTIEDSGINAKFVNAVYDTLLNT PQDVQKTVLKGIINSLLREWKGPRT KDDLRAYFILLQNPQFNNTSTYVIY AHLLRQIATLVEADHHFLVHWFKKL SQKRFKQLVERLLQFISLRLFPAKP EEFPPITKCSWWIPSAAKVLALLNT ANNLVHPPLIPYTDFYNSTLDHIDL MEEYHTWQNFGNSHRFSFCQYPFVI SVAAKKIIIQRDSEQQMINIARQSL VDKVSRRQRPDMNILFLNMKVRRTH LVSDSLDELTRKRADLKKKLKVTFV GEAGLDMGGLTKEWFLLLIRQIFHP DYGMFTYHKDSHCHWFSSFKCDNYS EFRLVGILMGLAVYNSITFDIRFPP CCYKKLLSPPIIPSGQNIPVGICNV TVDDLCQIMPELAHGLSELLSHEGN VEEDFYSTFQVFQEEFGIIKSYNLK PGGDKISVTNQNRKEYVQLYTDFLL NKSIYKQFAAFYYGFHSVCASNALM LLRPEEVEILVCGSPDLDMHALQRS TQYDGYAKTDLTIKYFWDVVLGFPL DLQKKLLHFTTGSDRVPVGGMADLN FKISKNETSTNCLPVAHTCFNQLCL PPYKSKKDLKQKLIIGISNSEGFGL ETRTRPLEQKLISEEDLAANDILDY KDDDDKV |
| Purity : |
> 80% as determined by SDS-PAGE and Coomassie blue staining |
| Stability : |
Stable for 3 months from receipt of products under proper storage and handling conditions. |
| Storage : |
Store at -80 centigrade. Avoid repeated freeze-thaw cycles. |
| Concentration : |
50 μg/mL as determined by BCA |
| Storage Buffer : |
100 mM glycine, 25 mM Tris-HCl, pH 7.3. |
