Recombinant Human HENMT1 Protein, MYC/DDK-tagged, C13 and N15-labeled

• Cat.No.: HENMT1-210H
• Product Overview: C1orf59 MS Standard C13 and N15-labeled recombinant protein (NP_653185) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: Methyltransferase that adds a 2'-O-methyl group at the 3'-end of piRNAs, a class of 24 to 30 nucleotide RNAs that are generated by a Dicer-independent mechanism and are primarily derived from transposons and other repeated sequence elements. This probably protects the 3'-end of piRNAs from uridylation activity and subsequent degradation. Stabilization of piRNAs is essential for gametogenesis.
• Source: HEK293
• Species: Human
• Tag: Myc/DDK
• Molecular Mass: 44.5 kDa
• AA Sequence: MEENNLQCSSVVDGNFEEVPRETAI QFKPPLYRQRYQFVKNLVDQHEPKK VADLGCGDTSLLRLLKVNPCIELLV GVDINEDKLRWRGDSLAPFLGDFLK PRDLNLTITLYHGSVVERDSRLLGF DLITCIELIEHLDSGDLARFPEVVF GYLSPSMIVISTPNSEFNPLFPSVT LRDSDHKFEWTRMEFQTWALYVANR YDYSVEFTGVGEPPAGAENVGYCTQ IGIFRKNGGKATESCLSEQHDQHVY KAVFTTSYPSLQQERFFKLVLVNEV SQQVESLRVSHLPRRKEQAGERGDK PKDIGGSKAPVPCFGPVFTEVEKAK IENSPTPFCVGDKFFVPLQRLLAYP KLNRLCANEEIMRSVIADSIPLSSD GSAVVADLRNYFDEQFEFTRTRPLE QKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: HENMT1 HEN1 methyltransferase homolog 1 (Arabidopsis) [ Homo sapiens (human) ]
• Official Symbol: HENMT1
• Synonyms: HEN1; C1orf59; RP11-256E16.2
• Gene ID: 113802
• mRNA Refseq: NM_144584
• Protein Refseq: NP_653185
• MIM: 612178
• UniProt ID: Q5T8I9