Recombinant Human HGD protein, His-tagged

• Cat.No.: HGD-1174H
• Product Overview: Recombinant Human HGD aa. (Val239~Glu428 (Accession # Q93099) fused with N-terminal His tag was produced in E. coli cells.

Specification

• Description: This gene encodes the enzyme homogentisate 1,2 dioxygenase. This enzyme is involved in the catabolism of the amino acids tyrosine and phenylalanine. Mutations in this gene are the cause of the autosomal recessive metabolism disorder alkaptonuria.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Freeze-dried powder
• Molecular Mass: 24kDa as determined by SDS-PAGE reducing conditions.
• Protein length: Val239~Glu428 (Accession # Q93099)
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: >95%
• Characteristic: The isoelectric point is 8.8.
• Applications: SDS-PAGE; WB; ELISA; IP.
• Stability: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: Supplied as lyophilized form in PBS, pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.

Gene Information

• Gene Name: HGD homogentisate 1,2-dioxygenase [ Homo sapiens (human) ]
• Official Symbol: HGD
• Synonyms: HGD; homogentisate 1,2-dioxygenase; AKU; HGO; homogentisate oxidase; homogentisate oxygenase; homogentisic acid oxidase; homogentisicase
• Gene ID: 3081
• mRNA Refseq: NM_000187.3
• Protein Refseq: NP_000178.2
• UniProt ID: Q93099