Recombinant Human HMCES Protein, Myc/DDK-tagged, C13 and N15-labeled
Cat.No. : | HMCES-2613H |
Product Overview : | C3orf37 MS Standard C13 and N15-labeled recombinant protein (NP_001006109) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells. |
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Description : | Sensor of abasic sites in single-stranded DNA (ssDNA) required to preserve genome integrity by promoting error-free repair of abasic sites. Acts as an enzyme that recognizes and binds abasic sites in ssDNA at replication forks and chemically modifies the lesion by forming a covalent cross-link with DNA: forms a stable thiazolidine linkage between a ring-opened abasic site and the alpha-amino and sulfhydryl substituents of its N-terminal catalytic cysteine residue. The HMCES DNA-protein cross-link is then degraded by the proteasome. Promotes error-free repair of abasic sites by acting as a 'suicide' enzyme that is degraded, thereby protecting abasic sites from translesion synthesis (TLS) polymerases and endonucleases that are error-prone and would generate mutations and double-strand breaks. Has preference for ssDNA, but can also accommodate double-stranded DNA with 3' or 5' overhang (dsDNA), and dsDNA-ssDNA 3' junction. Also involved in class switch recombination (CSR) in B-cells independently of the formation of a DNA-protein cross-link: acts by binding and protecting ssDNA overhangs to promote DNA double-strand break repair through the microhomology-mediated alternative-end-joining (Alt-EJ) pathway. Acts as a protease: mediates autocatalytic processing of its N-terminal methionine in order to expose the catalytic cysteine. |
Source : | HEK293 |
Species : | Human |
Tag : | Myc/DDK |
Molecular Mass : | 40.6 kDa |
AA Sequence : | MCGRTSCHLPRDVLTRACAYQDRRG QQRLPEWRDPDKYCPSYNKSPQSNS PVLLSRLHFEKDADSSERIIAPMRW GLVPSWFKESDPSKLQFNTTNCRSD TVMEKRSFKVPLGKGRRCVVLADGF YEWQRCQGTNQRQPYFIYFPQIKTE KSGSIGAADSPENWEKVWDNWRLLT MAGIFDCWEPPEGGDVLYSYTIITV DSCKGLSDIHHRMPAILDGEEAVSK WLDFGEVSTQEALKLIHPTENITFH AVSSVVNNSRNNTPECLAPVDLVVK KELRASGSSQRMLQWLATKSPKKED SKTPQKEESDVPQWSSQFLQKSPLP TKRGTAGLLEQWLKREKEEEPVAKR PYSQTRTRPLEQKLISEEDLAANDI LDYKDDDDKV |
Purity : | > 80% as determined by SDS-PAGE and Coomassie blue staining |
Stability : | Stable for 3 months from receipt of products under proper storage and handling conditions. |
Storage : | Store at -80 centigrade. Avoid repeated freeze-thaw cycles. |
Concentration : | 50 μg/mL as determined by BCA |
Storage Buffer : | 100 mM glycine, 25 mM Tris-HCl, pH 7.3. |
Gene Name : | HMCES 5-hydroxymethylcytosine binding, ES cell specific [ Homo sapiens (human) ] |
Official Symbol : | HMCES |
Synonyms : | HMCES; 5-hydroxymethylcytosine binding, ES cell specific; DC12; SRAPD1; C3orf37; abasic site processing protein HMCES; 5-hydroxymethylcytosine (hmC) binding, ES cell-specific; ES cell-specific 5hmC-binding protein; SOS response associated peptidase domain containing 1; SRAP domain-containing protein 1; UPF0361 protein C3orf37; embryonic stem cell-specific 5-hydroxymethylcytosine-binding protein; peptidase HMCES; putative endonuclease HMCES; putative peptidase SRAPD1 |
Gene ID : | 56941 |
mRNA Refseq : | NM_001006109 |
Protein Refseq : | NP_001006109 |
MIM : | 618288 |
UniProt ID : | Q96FZ2 |
Products Types
◆ Recombinant Protein | ||
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HMCES-5764Z | Recombinant Zebrafish HMCES | +Inquiry |
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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