Recombinant Human HNRNPK Protein (3-459 aa), GST-tagged

• Cat.No.: HNRNPK-1190H
• Product Overview: Recombinant Human HNRNPK Protein (3-459 aa) is produced by E. coli expression system. This protein is fused with a GST tag at the N-terminal. Protein Description: Partial.

Specification

• Description: One of the major pre-mRNA-binding proteins. Binds tenaciously to poly(C) sequences. Likely to play a role in the nuclear metabolism of hnRNAs, particularly for pre-mRNAs that contain cytidine-rich sequences. Can also bind poly(C) single-stranded DNA. Plays an important role in p53/TP53 response to DNA damage, acting at the level of both transcription activation and repression. When sumoylated, acts as a transcriptional coactivator of p53/TP53, playing a role in p21/CDKN1A and 14-3-3 sigma/SFN induction . As far as transcription repression is concerned, acts by interacting with long intergenic RNA p21 (lincRNA-p21), a non-coding RNA induced by p53/TP53. This interaction is necessary for the induction of apoptosis, but not cell cycle arrest.3 Publications.
• Source: E. coli
• Species: Human
• Tag: GST
• Form: Tris-based buffer,50% glycerol
• Molecular Mass: 77.8 kDa
• Protein length: 3-459 aa
• AA Sequence: TEQPEETFPNTETNGEFGKRPAEDM EEEQAFKRSRNTDEMVELRILLQSK NAGAVIGKGGKNIKALRTDYNASVS VPDSSGPERILSISADIETIGEILK KIIPTLEEGLQLPSPTATSQLPLES DAVECLNYQHYKGSDFDCELRLLIH QSLAGGIIGVKGAKIKELRENTQTT IKLFQECCPHSTDRVVLIGGKPDRV VECIKIILDLISESPIKGRAQPYDP NFYDETYDYGGFTMMFDDRRGRPVG FPMRGRGGFDRMPPGRGGRPMPPSR RDYDDMSPRRGPPPPPPGRGGRGGS RARNLPLPPPPPPRGGDLMAYDRRG RPGDRYDGMVGFSADETWDSAIDTW SPSEWQMAYEPQGGSGYDYSYAGGR GSYGDLGGPIITTQVTIPKDLAGSI IGKGGQRIKQIRHESGASIKIDEPL EGSEDRIITITGTQDQIQNAQYLLQ NSVKQYS
• Purity: > 90% as determined by SDS-PAGE.
• Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 centigrade for up to one week.
• Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 centigrade/-80 centigrade. The shelf life of lyophilized form is 12 months at -20 centigrade/-80 centigrade.
• Concentration: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Gene Information

• Gene Name: HNRNPK heterogeneous nuclear ribonucleoprotein K [ Homo sapiens ]
• Official Symbol: HNRNPK
• Synonyms: HNRNPK; HNRPK; CSBP; TUNP; FLJ41122
• Gene ID: 3190
• mRNA Refseq: NM_002140
• Protein Refseq: NP_002131
• MIM: 600712
• UniProt ID: P61978