Recombinant Human HRG protein(Met1-Lys525), His-tagged

• Cat.No.: HRG-753H
• Product Overview: Recombinant Human HPRG (NP_000403.1) (Met 1-Lys 525) with a C-terminal polyhistidine tag was expressed in HEK293.

Specification

• Source: HEK293
• Species: Human
• Tag: C-His
• Protein length: Met1-Lys525
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its ability to support the adhesion of MOLT-4 human acute lymphoblastic leukemia cells. (Lamb-Wharton, R.J. and W.T. Morgen, 1993, Cell Immunol. 152: 544). Human HPRG immobilized at 1 μg/ml (100 μl/well) will induce >65% MOLT4 cell adhesion (1 x 10E5 cells/well) in the presence of 7.5 μg/ml Concanvalin A.
• Molecular Mass: The secreted recombinant human HPRG comprises 518 amino acids with a predicted molecular mass of 59 kDa. As a result of glycosylation, rhHPRG migrates as an approximately 75-80 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 97 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
• Publication:
  Structure and Interactions of a Dimeric Variant of sHIP, a Novel Virulence Determinant of Streptococcus pyogenes. (2016)
  Functional and Structural Properties of a Novel Protein and Virulence Factor (sHIP) in Streptococcus pyogenes (2014)

Gene Information

• Gene Name: HRG histidine-rich glycoprotein [ Homo sapiens ]
• Official Symbol: HRG
• Synonyms: HRG; histidine-rich glycoprotein; histidine proline rich glycoprotein; HPRG; HRGP; thrombophilia due to elevated HRG; histidine-proline-rich glycoprotein; THPH11; DKFZp779H1622
• Gene ID: 3273
• mRNA Refseq: NM_000412
• Protein Refseq: NP_000403
• MIM: 142640
• UniProt ID: P04196