| Description : |
The protein encoded by this gene is a member of the heat shock protein 70 (HSP70) family. This protein localizes to the lumen of the endoplasmic reticulum (ER) where it operates as a typical HSP70 chaperone involved in the folding and assembly of proteins in the ER and is a master regulator of ER homeostasis. During cellular stress, as during viral infection or tumorogenesis, this protein interacts with the transmembrane stress sensor proteins PERK (protein kinase R-like endoplasmic reticulum kinase), IRE1 (inositol-requiring kinase 1), and ATF6 (activating transcription factor 6) where it acts as a repressor of the unfolded protein response (UPR) and also plays a role in cellular apoptosis and senescence. Elevated expression and atypical translocation of this protein to the cell surface has been reported in viral infections and some types of cancer cells. At the cell surface this protein may facilitate viral attachment and entry to host cells. This gene is a therapeutic target for the treatment of coronavirus diseases and chemoresistant cancers. |
| Source : |
E. coli |
| Species : |
Human |
| Tag : |
His |
| Form : |
Liquid |
| Molecular Mass : |
72.9 kDa (659aa) |
| Protein length : |
19-654 |
| AA Sequence : |
EEEDKKEDVGTVVGIDLGTTYSCVG VFKNGRVEIIANDQGNRITPSYVAF TPEGERLIGDAAKNQLTSNPENTVF DAKRLIGRTWNDPSVQQDIKFLPFK VVEKKTKPYIQVDIGGGQTKTFAPE EISAMVLTKMKETAEAYLGKKVTHA VVTVPAYFNDAQRQATKDAGTIAGL NVMRIINEPTAAAIAYGLDKREGEK NILVFDLGGGTFDVSLLTIDNGVFE VVATNGDTHLGGEDFDQRVMEHFIK LYKKKTGKDVRKDNRAVQKLRREVE KAKRALSSQHQARIEIESFYEGEDF SETLTRAKFEELNMDLFRSTMKPVQ KVLEDSDLKKSDIDEIVLVGGSTRI PKIQQLVKEFFNGKEPSRGINPDEA VAYGAAVQAGVLSGDQDTGDLVLLD VCPLTLGIETVGGVMTKLIPRNTVV PTKKSQIFSTASDNQPTVTIKVYEG ERPLTKDNHLLGTFDLTGIPPAPRG VPQIEVTFEIDVNGILRVTAEDKGT GNKNKITITNDQNRLTPEEIERMVN DAEKFAEEDKKLKERIDTRNELESY AYSLKNQIGDKEKLGGKLSSEDKET MEKAVEEKIEWLESHQDADIEDFKA KKKELEEIVQPIISKLYGSAGPPPT GEEDTAEKDEL |
| Purity : |
> 90% by SDS-PAGE |
| Applications : |
SDS-PAGE |
| Storage : |
Can be stored at +2 to +8 centigrade for 1 week. For long term storage, aliquot and store at -20 to -80 centigrade. Avoid repeated freezing and thawing cycles. |
| Concentration : |
1 mg/mL (determined by Bradford assay) |
| Storage Buffer : |
20mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 20% glycerol, 1mM DTT |
