Recombinant Human HTT protein, His-tagged

• Cat.No.: HTT-3873H
• Product Overview: Recombinant Human HTT protein(2845-3000 aa), fused to His tag, was expressed in E. coli.

Specification

• Source: E. coli
• Species: Human
• Tag: His
• Form: The purified protein was Lyophilized from sterile PBS (58mM Na2HPO4,17mM NaH2PO4, 68mM NaCl, pH7.4). 5 % trehalose and 5 % mannitol are added as protectant before lyophilization. The elution buffer contain 300mM imidazole.
• Protein length: 2845-3000 aa
• AA Sequence: LDVGPEFSASIIQMCGVMLSGSEES TPSIIYHCALRGLERLLLSEQLSRL DAESLVKLSVDRVNVHSPHRAMAAL GLMLTCMYTGKEKVSPGRTSDPNPA APDSESVIVAMERVSVLFDRIRKGF PCEARVVARILPQFLDDFFPPQDIM NKVIGE
• Purity: 90%, by SDS-PAGE with Coomassie Brilliant Blue staining.
• Storage: Short-term storage: Store at 2-8°C for (1-2 weeks).
  Long-term storage: Aliquot and store at -20°C to -80°C for up to 3 months, buffer containing 50% glycerol is recommended for reconstitution. Avoid repeat freeze-thaw cycles.
• Reconstitution: Reconstitute at 0.25 µg/μl in 200 μl sterile water for short-term storage.
  Reconstitution with 200 μl 50% glycerol solution is recommended for longer term storage.

Gene Information

• Gene Name: HTT huntingtin [ Homo sapiens ]
• Official Symbol: HTT
• Synonyms: HTT; huntingtin; HD, huntingtin (Huntington disease); IT15; huntington disease protein; HD
• Gene ID: 3064
• mRNA Refseq: NM_002111
• Protein Refseq: NP_002102
• MIM: 613004
• UniProt ID: P42858

Reviews

06/24/2021

This protein synthesis process is efficient and reliable, reducing experimental time and cost.

02/20/2021

This product has strict quality control and complies with relevant industry standards.

09/08/2020

When experiments were performed with this protein, the sensitivity and accuracy of the experiments were significantly improved.

Q&As

What diseases is HTT associated with?

HTT is associated with neurodegenerative diseases such as Huntington's disease, Parkinson's disease, and Alzheimer's disease.

How can animal models be used to study the function and disease mechanism of HTT protein?

Animal models carrying human mutant genes can be constructed using transgenic or gene editing technology to study the role of HTT protein in vivo and disease mechanisms. These animal models can be used to study the effects of HTT proteins on neuronal function, synaptic transmission, behavior, and more, as well as to evaluate the effects of potential therapeutic strategies.

What are the therapeutic strategies for HTT protein?

Therapeutic strategies for HTT protein include inhibiting its expression, reducing its activity, or changing its modification state. A number of small molecule inhibitors and gene therapy strategies targeting HTT proteins are currently being studied.

Is there a challenge in HTT protein research?

There are still some challenges in the research of HTT proteins, such as the need to better understand their structure and functional relationships, and develop more effective drugs or treatments. In addition, due to the complexity and individual differences of neurodegenerative diseases, therapeutic strategies targeting HTT proteins also need to be adapted and optimized according to individual conditions.

How does HTT protein interact with other proteins or signaling pathways?

HTT protein can interact with other proteins and signaling pathways. For example, HTT proteins can interact with transcription factors to affect gene transcription, and can also interact with signaling pathways such as MAPK (mitogen-activated protein kinase) to participate in cell proliferation and apoptosis.

How can HTT protein be used for disease diagnosis?

By measuring the expression level and modification status of HTT protein, it can be used to diagnose diseases such as Huntington's disease. At present, there are some detection methods for HTT protein, such as western blotting, immunohistochemistry, etc.