Recombinant Human HTT protein, MBP-His-tagged

• Cat.No.: HTT-1536H
• Product Overview: Recombinant Human HTT protein(Met1-Glu82), fused with His and MBP tag, was expressed in E.coli .

Specification

• Source: E.coli
• Species: Human
• Tag: MBP-His
• Form: Lyophilized from 0.22 μm filtered solution in 20 mM HEPES, 150 mM NaCl, pH7.5 with trehalose as protectant.
• Molecular Mass: This protein carries a MBP tag at the N-terminus and a polyhistidine tag at the C-terminusThe protein has a calculated MW of 52.7 kDa. The protein migrates as 60-63 kDa under reducing (R) condition (SDS-PAGE).
• Proteinlength: Met1-Glu82
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at:
  -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: HTT huntingtin [ Homo sapiens ]
• Official Symbol: HTT
• Synonyms: HTT; huntingtin; HD, huntingtin (Huntington disease); IT15; huntington disease protein; HD
• Gene ID: 3064
• mRNA Refseq: NM_002111
• Protein Refseq: NP_002102
• MIM: 613004
• UniProt ID: P42858

Reviews

06/24/2021

This protein synthesis process is efficient and reliable, reducing experimental time and cost.

02/20/2021

This product has strict quality control and complies with relevant industry standards.

09/08/2020

When experiments were performed with this protein, the sensitivity and accuracy of the experiments were significantly improved.

Q&As

What diseases is HTT associated with?

HTT is associated with neurodegenerative diseases such as Huntington's disease, Parkinson's disease, and Alzheimer's disease.

How can animal models be used to study the function and disease mechanism of HTT protein?

Animal models carrying human mutant genes can be constructed using transgenic or gene editing technology to study the role of HTT protein in vivo and disease mechanisms. These animal models can be used to study the effects of HTT proteins on neuronal function, synaptic transmission, behavior, and more, as well as to evaluate the effects of potential therapeutic strategies.

What are the therapeutic strategies for HTT protein?

Therapeutic strategies for HTT protein include inhibiting its expression, reducing its activity, or changing its modification state. A number of small molecule inhibitors and gene therapy strategies targeting HTT proteins are currently being studied.

Is there a challenge in HTT protein research?

There are still some challenges in the research of HTT proteins, such as the need to better understand their structure and functional relationships, and develop more effective drugs or treatments. In addition, due to the complexity and individual differences of neurodegenerative diseases, therapeutic strategies targeting HTT proteins also need to be adapted and optimized according to individual conditions.

How does HTT protein interact with other proteins or signaling pathways?

HTT protein can interact with other proteins and signaling pathways. For example, HTT proteins can interact with transcription factors to affect gene transcription, and can also interact with signaling pathways such as MAPK (mitogen-activated protein kinase) to participate in cell proliferation and apoptosis.

How can HTT protein be used for disease diagnosis?

By measuring the expression level and modification status of HTT protein, it can be used to diagnose diseases such as Huntington's disease. At present, there are some detection methods for HTT protein, such as western blotting, immunohistochemistry, etc.