Recombinant Human ICAM1 protein, Fc-tagged

• Cat.No.: ICAM1-655H
• Product Overview: Recombinant Human ICAM1 protein (Gln28-Glu480), fused to human IgG1 Fc tag at C-terminus, was expressed in human 293 cells (HEK293).

Specification

• Description: Inter-Cellular Adhesion Molecule 1 (ICAM-1) is also known as Cluster of Differentiation 54 (CD54), is a member of the immunoglobulin superfamily, and is a cell surface glycoprotein which is typically expressed in low concentrations on endothelial cells and cells of the immune system. The protein encoded by this gene is a type of intercellular adhesion molecule continuously present in low concentrations in the membranes of leukocytes and endothelial cells. Upon cytokine stimulation, the concentrations greatly increase. ICAM-1 can be induced by interleukin-1 (IL-1) and tumor necrosis factor alpha (TNFα) and is expressed by the vascular endothelium, macrophages, and lymphocytes. ICAM-1 is a ligand for LFA-1 (integrin), a receptor found on leukocytes. When activated, leukocytes bind to endothelial cells via ICAM-1/LFA-1 and then transmigrate into tissues. ICAM-1 has been implicated in subarachnoid hemorrhage (SAH). Levels of ICAM-1 are shown to be significantly elevated in patients with SAH over control subjects in many studies. ICAM-1 expressed by respiratory epithelial cells is also the binding site for rhinovirus, the causative agent of most common colds.
• Source: HEK293
• Species: Human
• Tag: human IgG1 Fc
• Form: Lyophilized from 0.22 um filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5, 10% trehalose.
• Molecular Mass: The protein has a calculated MW of 75.7 kDa. The protein migrates as 90-120 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Protein length: 453
• Endotoxin: Less than 1.0 EU per ug by the LAL method.
• Purity: >98% as determined by SDS-PAGE.
• Storage: For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
  Please avoid repeated freeze-thaw cycles.
  This product is stable after storage at:
  -20 centigrade to -70 centigrade for 12 months in lyophilized state;
  -70 centigrade for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: ICAM1
• Official Symbol: ICAM1
• Synonyms: ICAM1; intercellular adhesion molecule 1; BB2; CD54; human rhinovirus receptor; ICAM-1; cell surface glycoprotein P3.58; major group rhinovirus receptor; intercellular adhesion molecule 1 (CD54), human rhinovirus receptor; P3.58
• Gene ID: 3383
• mRNA Refseq: NM_000201
• Protein Refseq: NP_000192
• MIM: 147840
• UniProt ID: P05362

Reviews

04/27/2022

Manufacturers may provide documentation of the protein's validation, which includes information about its purity, concentration, stability, and potential contaminants.

11/05/2020

This can be particularly important in clinical trials to ensure accurate and reliable results.

10/14/2016

If the need arises for larger quantities of ICAM1 protein during the later stages of clinical trials, the manufacturer can facilitate scale-up production processes to meet those requirements.

Q&As

Can ICAM1 be a potential therapeutic target?

Yes, targeting ICAM1 is being explored as a potential therapeutic strategy for inflammatory diseases, with research focusing on developing ICAM1 inhibitors.

How is ICAM1 involved in inflammation?

ICAM1 is upregulated during inflammation, promoting the adhesion of immune cells to the endothelium and facilitating their migration into inflamed tissues.

How is ICAM1 involved in cancer progression?

ICAM1 expression can be altered in cancer cells and is implicated in promoting cancer progression by facilitating tumor cell adhesion, invasion, and metastasis.

Are there diagnostic applications for ICAM1 in clinical settings?

ICAM1 levels can be measured in blood or tissue samples, and elevated levels may serve as diagnostic markers for certain inflammatory diseases.

Can ICAM1 be used as a prognostic marker in certain diseases?

Elevated levels of ICAM1 in some diseases may serve as prognostic markers, indicating disease severity and potential outcomes.